Related Experiment Video
Updated: Aug 16, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
Evidence for the presence of cAMP, cAMP receptor and transcription termination factor rho in different gram-negative
Abstract:
Cyclic AMP has been shown to be present in 12 different Gram-negative bacteria and the regulation of its concentration, as a function of growth conditions, is similar to that described for Escherichia coli K12. Antibodies raised against catabolite activator protein (CAP) and Rho protein of E. coli K12 were used to check for the occurrence of cross-reactive antigens. Using radioimmunological assays, immunoblotting techniques and biochemical criteria we showed a wide distribution of CAP and Rho, structurally and functionally closely related to the corresponding E. coli K12 proteins. These results suggest that transcription is similarly regulated by these factors in Gram-negative bacteria.
Related Concept Videos
Bacterial RNA Polymerase
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Transcription Attenuation in Prokaryotes
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Transcription Initiation
The promoters and enhancers and their accessory proteins allow tight regulation of...
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Transcription in Prokaryotes

