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Updated: Feb 7, 2026

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
Published on: June 30, 2017
Hispidulin inhibits adipogenesis in 3T3-L1 adipocytes through PPARγ pathway
Seul Gi Lee1, Jin Soo Kim1, Kyoungjin Min2
1Department of Food Science and Biotechnology, Kyungpook National University, Daegu, 41566, Republic of Korea.
Abstract:
Hispidulin, a natural flavone, has been reported to have diverse pharmacological effects, including antifungal, antioxidant, and antithrombotic properties. However, an anti-adipogenic effect has not yet been reported, which is the focus of the current study. Hispidulin suppressed the differentiation of adipocytes and cellular lipid accumulation without cytotoxicity. Treatment with hispidulin at concentrations of 10, 20, and 40 μM reduced intracellular lipids by 88.1%, 81.9%, and 75.8%, respectively. In addition, hispidulin reduced mRNA and protein expression of peroxisome proliferator-activated receptor gamma (PPARγ) and adiponectin. To our knowledge, these results are the first evidence of the anti-adipogenic effects of hispidulin in 3T3-L1 adipocytes, indicating that hispidulin has potential as a novel anti-obesity therapeutic.
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