Time-lapse imaging beyond the diffraction limit
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Updated: Feb 7, 2026

Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing
Published on: April 25, 2019
Ajay Chitnis1, Damian Dalle Nogare2
1Eunice Kennedy Shriver National Institute of Child Health and Human Development, United States.
Abstract:
The zebrafish, with its rapid external development, optical transparency, and the relative ease with which transgenic lines can be created, is rapidly becoming the model of choice for examining developmental processes via time-lapse microscopy. The recent proliferation of techniques for super-resolution imaging now allows for an unprecedented view of embryonic development at high spatial and temporal resolution in live tissues. This review examines both the theoretical basis and practical application of a number of established and emerging super-resolution microscopy techniques, focusing on their application in time-lapse imaging of live zebrafish embryos.
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