Related Experiment Video
Updated: Feb 7, 2026

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
Published on: October 30, 2016
Sensitive detection of Campylobacter jejuni using one-step strategy based on functional nanospheres of immunomagnetic
Kuo He1, Xiuyuan Zhang2, Lin Chen3
1Food Safety Centre, Hebei North University, Zhangjiakou 075000, China.
Abstract:
Campylobacter jejuni has emerged as the most common bacterial foodborne illness in the developed world. Here, we demonstrate a convenient one-step strategy for detecting C. jejuni. Immunomagnetic nanospheres (IMNS) and immunofluorescent nanospheres (IFNS, quantum dots) were used for the simultaneous, sensitive capture and recognition of C. jejuni. After magnetic separation with the IMNS, detection of C. jejuni was achieved with fluorescence measurement of the IFNS in the sandwich complexes (IMNS-bacteria-IFNS). The limit of detection of this assay was 103 CFU/mL, and the linear range was from 105 to 107 CFU/mL (R2 = 0.9994). When compared with a conventional two-step detection strategy, in which C. jejuni was first captured with the IMNS and then detected using the IFNS, this one-step detection strategy enhance sensitivity and save time. This suggested that the developed method has the potential for use as an alternative to the standard method for food quality assurance, as it provides rapid detection of C. jejuni in foodstuffs and the environment.
More Related Videos
Related Concept Videos
Quantum Numbers
The Quantum-Mechanical Model of an Atom
The Dot Product
Dot Product
In engineering, the dot product of any two vectors is the product of the magnitudes of the vectors and the cosine of the angle between them. It is denoted by a dot symbol between the two vectors.
Consider a vehicle pulling an object along the ground using a rope. If the rope makes an angle with the horizontal axis, the work done can be calculated using the dot product of the force applied and the object's displacement.
The dot...
Exponential Functions with Base e
Dot Product: Problem Solving
Identify the problem: Start by reading the problem and...

