[Restriction analysis of DNA from phage SM of Pseudomonas aeruginosa]

The DNA of temperate phage SM P. aeruginosa has one PvuII site, two BamHI sites, three HindIII sites and five EcoRI sites. Using these restrictases the physical map of the phage genome has been constructed. The DNA of phage SM has in their structure cohesive ends similar to cos-sites of phage lambda DNA. EcoRI-fragments with cohesive ends have molecular masses 2.9 and 4.9 MDa.