Related Experiment Video
Updated: Feb 7, 2026

Choice and No-Choice Assays for Testing the Resistance of A. thaliana to Chewing Insects
Published on: May 14, 2008
Two choices good, four choices better: For measuring stereoacuity in children, a four-alternative forced-choice
Kathleen Vancleef1, Jenny C A Read1, William Herbert1
1Institute of Neuroscience, Newcastle University, Newcastle-upon-Tyne, United Kingdom.
Purpose:
Measuring accurate thresholds in children can be challenging. A typical psychophysical experiment is usually too long to keep children engaged. However, a reduction in the number of trials decreases the precision of the threshold estimate. We evaluated the efficiency of forced-choice paradigms with 2 or 4 alternatives (2-AFC, 4-AFC) in a disparity detection experiment. 4-AFC paradigms are statistically more efficient, but also more cognitively demanding, which might offset their theoretical advantage in young children.
Methods:
We ran simulations evaluating bias and precision of threshold estimates of 2-AFC and 4-AFC paradigms. In addition, we measured disparity thresholds in 43 children (aged 6 to 17 years) with a 4-AFC paradigm and in 49 children (aged 4 to 17 years) with a 2-AFC paradigm, both using an adaptive weighted one-up one-down staircase.
Results:
Simulations indicated a similar bias and precision for a 2-AFC paradigm with double the number of trials as a 4-AFC paradigm. On average, estimated threshold of the simulated data was equal to the model threshold, indicating no bias. The precision was improved with an increasing number of trials. Likewise, our data showed a similar bias and precision for a 2-AFC paradigm with 60 trials as for a 4-AFC paradigm with 30 trials. Trials in the 4-AFC paradigm took slightly longer as participants scanned more alternatives. However, the 4-AFC task still ended up faster for a given precision.
Conclusion:
Bias and precision were similar in a 4-AFC task compared to a 2-AFC task with double the number of trials. However, a 4-AFC paradigm was more time efficient and is therefore recommended.
Related Concept Videos
Mate Choice
Personal Choice and Fate Attributions
Factors Affecting α-Alkylation of Ketones: Choice of Base
The reaction involving bases like EtO− whose conjugate acid EtOH (pKa = 15.9) is stronger than the ketone (pKa = 19.2) results in an equilibrium mixture with higher ketone concentration. As a consequence,...
Alternative RNA Splicing
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing
Electromotive Force

