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Related Experiment Videos

A human DNA-binding protein is methylation-specific and sequence-specific.

R Y Wang, X Y Zhang, M Ehrlich

    Nucleic Acids Research
    |February 25, 1986
    PubMed
    Summary
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    Methylated DNA-binding protein (MDBP) specifically binds to methylated DNA sequences. This protein

    Area of Science:

    • Molecular Biology
    • Epigenetics
    • Human Genetics

    Background:

    • DNA methylation is a critical epigenetic mechanism regulating gene expression.
    • Methylated DNA-binding proteins (MDBPs) are involved in recognizing methylated DNA.
    • The specific DNA sequences recognized by MDBP are not fully understood.

    Purpose of the Study:

    • To investigate the sequence-specific DNA binding of human MDBP.
    • To determine if MDBP binding is dependent on DNA methylation.
    • To identify the specific DNA sequence recognized by MDBP.

    Main Methods:

    • Isolation of MDBP from human placenta.
    • Methylation of pBR322 DNA fragments using human DNA methyltransferase.
    • DNA binding assays to identify MDBP-interacting fragments.

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  • DNase I footprinting to determine the specific MDBP-binding sequence.

    • Main Results:

    • MDBP exhibits both methylation-specific and sequence-specific DNA binding.
    • A specific 22-base sequence within a methylated DNA fragment was preferentially bound by MDBP.
    • MDBP binding requires the presence of 5-methylcytosine in the DNA.

    Conclusions:

    • Human MDBP is a sequence-specific DNA-binding protein that recognizes methylated DNA.
    • MDBP plays a role in mediating the functional consequences of DNA methylation in humans.
    • This finding contributes to understanding the mechanisms of epigenetic regulation in human cells.