Production and flocculating properties of a compound biopolymer flocculant from corn ethanol wastewater

A compound biopolymer flocculant (CBF) produced using corn ethanol wastewater as substrate was investigated. After optimization of culture conditions, 3.08 g/L of purified CBF was extracted from the culture broth following 48 h of cultivation. The CBF macromolecule is mainly composed of protein (15.9%) and polysaccharide (81.8%). The polysaccharide component includes neutral sugars (28.92%), amino sugars (4.04%) and uronic acid (11.69%), with the neutral sugars being glucose, mannose, and lactose at a molar ratio of 4.1:1.5:1.9. CBF is pH tolerant from 3.0 to 12.0 and thermal tolerant from 20 to 100 °C, allowing for its application over a wide range of conditions. Furthermore, the Langmuir model better describes CBF adsorption on kaolin clay, as compared to the Freundlich model. Charge neutralization and bridging mechanisms are the primary flocculation mechanisms. In addition, CBF shows a high methylene blue removal efficiency. These results indicate that this compound biopolymer flocculant has great potential in dye wastewater treatment.