Monoclonal antibodies to pseudorabies virus produced by mouse hybridomas

Monoclonal antibodies to pseudorabies viral capsid protein were prepared by fusing Sp2/0-Ag14 myeloma cells with pseudorabies virus (PrV)-primed Balb/c splenocytes. These monoclonal antibodies were specific for PrV. No crossreaction with Herpes Simplex Virus (HSV, type I) or infectious bovine rhinotrachitis virus (IBR) was found. The reaction between these monoclonal antibodies and PrV was demonstrated by immunofluorescent staining technique and enzyme-linked immunoblotting assay. These antibodies could be a useful diagnostic reagent for PrV as well as a good tool for the study of the capsid protein of PrV.