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Comparative iTRAQ proteomics revealed proteins associated with horn development in yak
Mingna Li1, Xiaoyun Wu1, Xian Guo1
1Key Laboratory for Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, 730050 People's Republic of China.
Proteome Science
|August 1, 2018
Summary
Selective breeding for hornless yak identified key proteins regulating horn development. This study reveals proteins involved in cell adhesion and metabolism are crucial for horn bud formation in polled yak.
Area of Science:
- Animal Genetics
- Proteomics
- Developmental Biology
Background:
- Dehorning yak poses safety risks, leading to selective breeding for genetically hornless (polled) yak.
- While the POLLED locus is known, the specific proteins regulating yak horn bud development remain largely uncharacterized.
Purpose of the Study:
- To identify and characterize differentially abundant proteins (DAPs) involved in horn bud development in polled versus horned yak fetuses.
- To elucidate the molecular mechanisms underlying horn development through proteomic analysis.
Main Methods:
- Differential proteomic analysis using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with 2D LC-MS/MS.
- Comparison of skin from the horn bud region of polled yak fetuses with horn bud tissue from horned yak fetuses.
Main Results:
- Identified 100 differentially abundant proteins (DAPs): 29 up-regulated and 71 down-regulated in polled yak fetuses.
- Up-regulated DAPs were primarily linked to metabolic activities.
- Down-regulated DAPs were significantly enriched in cell adhesion and cell movement pathways.
Conclusions:
- Proteins associated with cell adhesion, motility, keratinocyte differentiation, cytoskeleton organization, osteoblast differentiation, and fatty acid metabolism are important in yak horn bud development.
- Findings advance the understanding of the molecular basis of horn development in yak, particularly in polled breeds.
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