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Antimicrobial efficiency and stability of two decontamination solutions
Ingrida Smeringaiova1,2,3, Otakar Nyc4, Peter Trosan1,2,3
1Laboratory of the Biology and Pathology of the Eye, Department of Paediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic.
Cell and Tissue Banking
|August 1, 2018
Summary
Two decontamination solutions, BASE•128 and laboratory decontamination solution (LDS), show similar antimicrobial efficiency for placental tissues. LDS demonstrated superior efficacy against E. coli and E. faecalis, with minor stability differences observed.
Area of Science:
- Microbiology
- Biotechnology
- Pharmaceutical Science
Background:
- Decontamination solutions are crucial for tissue processing.
- BASE•128 and laboratory decontamination solution (LDS) contain similar antimicrobial agents.
- Assessing their comparative efficacy and stability is essential for clinical application.
Purpose of the Study:
- To compare the antimicrobial efficiency of BASE•128 and LDS against common human pathogens.
- To evaluate the stability of both solutions concerning pH and osmolarity after cold storage.
- To determine their suitability for decontaminating placental tissues.
Main Methods:
- Agar well diffusion assay was employed to test antimicrobial activity.
- Five human pathogens were used: Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli, and Enterococcus faecalis.
- pH and osmolarity were measured after 1, 3, and 6 months of cold storage.
Main Results:
- Both BASE•128 and LDS exhibited comparable antimicrobial efficacy against most tested pathogens.
- LDS showed significantly higher decontamination efficacy against E. coli and E. faecalis.
- LDS exhibited decreased osmolarity and increased pH after 6 months of cold storage, indicating reduced stability compared to BASE•128.
Conclusions:
- BASE•128 and LDS are similarly effective in eliminating potential bacterial contaminants from placental tissues.
- LDS demonstrates enhanced efficacy against specific pathogens like E. coli and E. faecalis.
- While BASE•128 shows better stability, both solutions may be considered for tissue decontamination, with potential considerations for LDS's stability over extended storage.
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