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Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain
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Strategy for peptide quantification using LC-MS in regulated bioanalysis: case study with a glucose-responsive

Yang Xu1, Dina Goykhman1, Ming Wang1

  • 1Pharmacokinetics, Pharmacodynamics & Drug Metabolism, Regulated Bioanalysis, Merck & Co., Inc., 770 Sumneytown Pike, WP75B-300, West Point, PA 19486, USA.

Bioanalysis
|August 1, 2018
PubMed
Summary

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) offers advanced peptide quantification but faces challenges. This study presents a validated LC-MS/MS strategy for hybrid assays, exemplified by glucose-responsive insulin (MK-2640).

Keywords:
GRIglucose-responsive insulinhybrid assaypeptide quantification

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