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Updated: Feb 7, 2026
Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells
Published on: February 23, 2011
Alka(e)ne synthesis in Cupriavidus necator boosted by the expression of endogenous and heterologous
Lucie Crépin1, Manon Barthe1, Florence Leray1
1LISBP, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France.
Abstract:
To boost aldehyde deformylating oxygenase (ADO) activity in a Cupriavidus necator strain expressing a synthetic alkane pathway, the expression of two ferredoxin-ferredoxin reductase systems was tested. The genes of a native fd/FNR-like system were identified in C. necator and expressed in a previously engineered alka(e)ne producing strain. The improved production of alka(e)nes in this Re2061-pMAB1 strain confirmed the activity of the native Fd/FNR system in C. necator. Concomitantly, the expression of the heterologous system from Synechococcus elongatus was investigated identically, leading to a second strain, Re2061-pMAB2. In the bioreactor, the aldehyde production was strongly reduced compared with the original alka(e)ne producer, leading to alka(e)nes production up to 0.37 and 1.48 g/L (22 and 82 mg/gCDW ), respectively. The alka(e)ne production yield of Re2061-pMAB2 accounted for 15% of the theoretical yield. We report here the highest level and yield of alka(e)nes production by an engineered bacterium to date.
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