Expression profiling and functional characterization of CD36 in turbot (Scophthalmus maximus L.)

CD36 is a scavenger receptor, a type of membrane-bound receptors that characterized by recognizing a variety of ligands including endogenous proteins and pathogens. Here, we characterized CD36 gene in turbot, and its expression patterns in mucosal barriers following different bacterial infection, as well as microbial ligand binding ability and bacteriostatic activities. In current study, one SmCD36 gene was captured with a 1407 bp open reading frame (ORF). In multiple species comparison, SmCD36 showed the highest similarity and identity to Cynoglossus semilaevis. In the phylogenetic analysis, SmCD36 showed the closest relationship to C. semilaevis, followed by Takifugu rubripes. The genomic structure analysis showed that CD36 had 12 exons with almost the same length in vertebrate species, indicating the conservation of CD36 during evolution. The syntenic analysis revealed that CD36 located between GNAI1 and SEMA3C genes across all the selected species, which suggested the synteny encompassing CD36 region during vertebrate evolution. Subsequently, SmCD36 was expressed in all the examined turbot tissues, with the highest expression level in intestine. In addition, SmCD36 was significantly up-regulated in intestine following both Gram-negative bacteria Vibrio anguillarum, and Gram-positive bacteria Streptococcus iniae immersion challenge. Finally, the rSmCD36 showed strong binding ability to all the examined microbial ligands and significant inhibition effect on Staphylococcus aureusrequires. Taken together, our results suggested SmCD36 involved in fish innate immune responses to bacterial infection. However, the knowledge of CD36 are still limited in teleost species, further studies should be carried out to better characterize its detailed roles in teleost mucosal immunity.