Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

9.3K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
9.3K
DNA as a Genetic Template02:05

DNA as a Genetic Template

27.9K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
27.9K
Genetics of Speciation02:16

Genetics of Speciation

21.7K
Speciation is the evolutionary process resulting in the formation of new, distinct species—groups of reproductively isolated populations.
21.7K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

10.0K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.0K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.7K
3.7K
Self Within Cultural Contexts01:30

Self Within Cultural Contexts

240
Cultural frameworks for understanding the self are often categorized into two broad orientations: individualism and collectivism. These paradigms influence how people define themselves, relate to others, and interpret their social worlds. Each orientation offers distinct perspectives on autonomy, responsibility, and the role of the individual within a community.Individualistic CulturesIn individualistic cultures like North America and Western Europe, identity is understood as autonomous and...
240

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteolysis activity mapping and substrate discovery platform for identifying tumor-activated biosensors.

Nature chemical biology·2026
Same author

Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.

Chemical reviews·2026
Same author

A Two-Protein Chemoreceptor Complex Regulates Oxygen Thresholds in Bacterial Magneto-Aerotaxis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Engineering strategies in bio-photoelectrochemical cells for sustainable energy and environmental applications.

Chemical communications (Cambridge, England)·2025
Same author

Streamlining tRNA-Synthetase Evolution for Genetic Code Expansion and Deep Sequencing Analyses of Its Evolved Variants.

ACS synthetic biology·2025
Same author

Hyperspectral reporters for long-distance and wide-area detection of gene expression in living bacteria.

Nature biotechnology·2025

Related Experiment Video

Updated: Feb 7, 2026

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
13:11

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

2.0K

Context effects of genetic code expansion by stop codon suppression.

Yonatan Chemla1, Eden Ozer1, Itay Algov1

  • 1Department of Life Sciences and Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, PO Box 653, Beer-Sheva 8410501, Israel.

Current Opinion in Chemical Biology
|August 1, 2018
PubMed
Summary
This summary is machine-generated.

Genetic code expansion allows incorporating unnatural amino acids into proteins. Stop codon suppression efficiency is influenced by mRNA context, but the underlying mechanisms require further investigation.

More Related Videos

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
07:26

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Published on: December 26, 2020

4.4K
Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers
10:41

Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers

Published on: June 24, 2019

8.8K

Related Experiment Videos

Last Updated: Feb 7, 2026

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
13:11

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

2.0K
Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
07:26

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Published on: December 26, 2020

4.4K
Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers
10:41

Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers

Published on: June 24, 2019

8.8K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • Genetic code expansion (GCE) allows unnatural amino acids (uaas) in proteins.
  • Stop codon suppression is a primary GCE method, enabling protein functionalization.
  • Observed variations in suppression efficiency are linked to cellular, operon, and mRNA contexts.

Purpose of the Study:

  • To review current knowledge on mRNA context effects in stop codon suppression.
  • To identify gaps in understanding the mechanisms governing these context effects.
  • To stimulate research and debate on advancing GCE technology.

Main Methods:

  • Review of existing literature on genetic code expansion and stop codon suppression.
  • Analysis of accumulated observations on factors influencing suppression efficiency.
  • Synthesis of current understanding and identification of knowledge gaps.

Main Results:

  • Stop codon suppression efficiency is significantly affected by mRNA context, particularly stop codon location.
  • Despite its importance, the precise mechanisms driving these context effects are largely unknown.
  • Numerous studies demonstrate the utility of GCE, highlighting the need to understand its limitations.

Conclusions:

  • Understanding mRNA context effects is crucial for optimizing genetic code expansion.
  • Further systematic research is needed to elucidate the mechanisms behind context-dependent suppression.
  • Addressing these knowledge gaps will accelerate the development and application of GCE technologies.