Related Experiment Video

Updated: Aug 16, 2026

High Precision Zinc Isotopic Measurements Applied to Mouse Organs
07:04

High Precision Zinc Isotopic Measurements Applied to Mouse Organs

Published on: May 22, 2015

Quantitation of zinc in nitric acid-digested plasma by atomic absorption spectrophotometry

R B Passey, K C Maluf, R Fuller

    Analytical Biochemistry
    |December 1, 1985
    PubMed

    More Related Videos

    Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells13:04

    Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells

    Published on: May 16, 2019

    Assessing Mineral Availability in Fish Feeds using Complementary Methods Demonstrated with the Example of Zinc in Atlantic Salmon04:54

    Assessing Mineral Availability in Fish Feeds using Complementary Methods Demonstrated with the Example of Zinc in Atlantic Salmon

    Published on: October 29, 2021

    Related Concept Videos

    EDTA: Auxiliary Complexing Reagents01:26

    EDTA: Auxiliary Complexing Reagents

    EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
    Precipitation Gravimetry01:03

    Precipitation Gravimetry

    Precipitation gravimetry is based on converting an analyte into a sparingly soluble precipitate, which is separated by filtration and weighed. An ideal precipitate should be pure, insoluble, of known composition, and easily filtered from the reaction mixture.
    In determining nickel by gravimetric analysis, a precipitant of ethanolic dimethylglyoxime is added to a hot nickel salt solution. This is quickly followed by the dropwise addition of dilute ammonia solution until precipitation occurs. A...
    Atomic Absorption Spectroscopy: Overview01:27

    Atomic Absorption Spectroscopy: Overview

    Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
    When irradiated by EMR of a particular wavelength, these...
    Atomic Absorption Spectroscopy: Atomization Methods01:25

    Atomic Absorption Spectroscopy: Atomization Methods

    Atomic Absorption Spectroscopy (AAS) atomizes samples through flame atomization or electrothermal atomization. Flame atomization typically involves a nebulizer and spray chamber assembly to combine the sample with a fuel–oxidant mixture, creating a fine aerosol mist that enters a burner. Typically, the fuel and oxidant are combined in an approximately stoichiometric ratio. However, for atoms that are easily oxidized, a fuel-rich mixture may be more advantageous. Only about 5% of the aerosol...
    Atomic Absorption Spectroscopy: Lab01:21

    Atomic Absorption Spectroscopy: Lab

    For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
     Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
    Atomic Emission Spectroscopy: Lab01:29

    Atomic Emission Spectroscopy: Lab

    AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...