Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

58.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.0K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.1K
Review and Preview01:10

Review and Preview

8.4K
In statistics, several tools are used to interpret the data. Measures of central tendency represent the characteristics of the data, such as mean, median, and mode. Additionally, measures of variance like standard deviation and range are used to find the spread of data from the mean. Relative standing measures the distance between data locations. Commonly used measures of relative standings are percentile, z score, and quartiles.
Percentiles are a type of fractile that partition data into...
8.4K
Review and Preview01:13

Review and Preview

11.5K
Data are individual items of information obtained from a population or sample. Data may be classified as qualitative (categorical), quantitative continuous, or quantitative discrete. Because it is not practical to measure the entire population in a study, researchers use samples to represent the population. A random sample is a representative group from the population chosen by using a method that gives each individual in the population an equal chance of being included in the sample. Random...
11.5K
Improving Translational Accuracy02:07

Improving Translational Accuracy

3.7K
3.7K
Improving Translational Accuracy02:07

Improving Translational Accuracy

15.0K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
15.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Caffeic acid prevents UVB radiation induced photocarcinogenesis through regulation of PTEN signaling in human dermal fibroblasts and mouse skin" [Toxicology and Applied Pharmacology, Volume 352, (2018) 87-96].

Toxicology and applied pharmacology·2026
Same author

Protocol for spatial profiling of immune cells in the mouse cornea and conjunctiva using NanoString GeoMx DSP and nCounterPro platforms.

STAR protocols·2026
Same author

Breeding vegetables for whitefly resistance: past, present, and future in the AI era.

Frontiers in plant science·2026
Same author

Artificial intelligence approaches for non-invasive diabetes prediction using ECG signals: A systematic review.

Computer methods and programs in biomedicine·2026
Same author

Correction: 'Learning from nature: photosynthetic traits conferring superior salt tolerance in wild rice <i>Oryza coarctata</i>' (2025), by Ping <i>et al.</i>

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2025
Same author

Epigenome editing for herbicide-resistant crops.

Trends in plant science·2025

Related Experiment Video

Updated: Feb 7, 2026

Cereal Crop Ear Counting in Field Conditions Using Zenithal RGB Images
11:49

Cereal Crop Ear Counting in Field Conditions Using Zenithal RGB Images

Published on: February 2, 2019

9.9K

CRISPR for Crop Improvement: An Update Review.

Deepa Jaganathan1, Karthikeyan Ramasamy1, Gothandapani Sellamuthu1

  • 1Plant Molecular Biology Laboratory, Department of Biotechnology, M. S. Swaminathan Research Foundation, Chennai, India.

Frontiers in Plant Science
|August 2, 2018
PubMed
Summary

CRISPR/Cas9 genome editing offers a precise and efficient method for crop improvement. This technology enables the development of non-GMO crops with enhanced resistance to biotic and abiotic stresses, leading to increased yield potential.

Keywords:
CRISPRTALENZFNabiotic stressbiotic stressquantitative trait loci

More Related Videos

Measuring Crop Motility and Food Passaging in Drosophila
06:13

Measuring Crop Motility and Food Passaging in Drosophila

Published on: May 9, 2020

6.3K
The Participant-Reported Implementation Update and Score PRIUS: A Novel Method for Capturing Implementation-Related Data Over Time
06:05

The Participant-Reported Implementation Update and Score PRIUS: A Novel Method for Capturing Implementation-Related Data Over Time

Published on: February 19, 2021

1.8K

Related Experiment Videos

Last Updated: Feb 7, 2026

Cereal Crop Ear Counting in Field Conditions Using Zenithal RGB Images
11:49

Cereal Crop Ear Counting in Field Conditions Using Zenithal RGB Images

Published on: February 2, 2019

9.9K
Measuring Crop Motility and Food Passaging in Drosophila
06:13

Measuring Crop Motility and Food Passaging in Drosophila

Published on: May 9, 2020

6.3K
The Participant-Reported Implementation Update and Score PRIUS: A Novel Method for Capturing Implementation-Related Data Over Time
06:05

The Participant-Reported Implementation Update and Score PRIUS: A Novel Method for Capturing Implementation-Related Data Over Time

Published on: February 19, 2021

1.8K

Area of Science:

  • Agricultural biotechnology
  • Molecular biology
  • Plant genetics

Background:

  • Genome sequencing and advanced breeding techniques enable targeting desirable crop traits.
  • Previous genome editing tools like ZFNs and TALENs are effective but complex and costly.
  • CRISPR/Cas9 offers a simpler, more versatile alternative for targeted gene modification.

Purpose of the Study:

  • To review CRISPR/Cas9 applications for crop improvement.
  • To highlight its use in enhancing stress tolerance in plants.
  • To discuss the development of non-GMO crops with improved traits.

Main Methods:

  • Utilizing CRISPR/Cas9 technology for precise genome editing in crops.
  • Employing modified Cas9 variants (e.g., Nmcas9, Sacas9, Stcas9) to enhance specificity and reduce off-target effects.
  • Leveraging Cas9 enzymes from diverse bacterial species for improved editing efficiency.

Main Results:

  • CRISPR/Cas9 has been successfully demonstrated for targeted gene modification in various crop plants.
  • Modified Cas9 systems and diverse Cas9 enzymes improve specificity and efficiency of genome editing.
  • Studies show successful application of CRISPR/Cas9 for enhancing biotic and abiotic stress tolerance.

Conclusions:

  • CRISPR/Cas9 provides plant biotechnologists with powerful tools for crop enhancement.
  • This technology facilitates the development of non-GMO crops with improved stress resistance and yield potential.
  • CRISPR/Cas9-mediated crop improvement is crucial for addressing agricultural challenges under environmental stresses.