Related Experiment Video
Updated: Feb 7, 2026

High-Throughput Measurement and Classification of Organic P in Environmental Samples
Published on: June 8, 2011
Direct Detection of Carbapenem-Resistant Organisms from Environmental Samples Using the GeneXpert Molecular
K A Perry1, J B Daniels2, S C Reddy2
1Division of Healthcare Quality Promotion, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA KPerry@cdc.gov.
Abstract:
In this pilot study, traditional culture and PCR methods were compared to the Cepheid GeneXpert IV molecular diagnostic system with the Xpert Carba-R assay (Carba-R assay) for detection of carbapenem resistance genes in primary environmental samples collected during a health care-related outbreak. Overall, traditional culture-dependent PCR and the Carba-R assay demonstrated 75% agreement. The Carba-R assay detected carbapenemase genes in five additional samples and in two samples that had additional genes when compared to culture-dependent PCR. The Carba-R assay could be useful for prioritizing further testing of environmental samples during health care-related outbreaks.IMPORTANCE Use of the Carba-R assay for detection of carbapenem-resistant Gram-negative organisms (CROs) can provide data for implementation of a rapid infection control response to minimize the spread of CROs in the health care setting.
Related Concept Videos
Resistivity
Resistance
Kinetic Molecular Theory: Molecular Velocities, Temperature, and Kinetic Energy
Types of Genetic Transfer Between Organisms
Molecular Models
Molecular Shape and Polarity

