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Micro-RNAs -106a and -362-3p in Peripheral Blood of Inflammatory Bowel Disease Patients
Ameneh Omidbakhsh1, Mohsen Saeedi2, Masoud Khoshnia3
1Student Research Committee, Gorgan Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Golestan province, Iran.
Objective:
MicroRNAs (miRNAs) can regulate various genes after binding to target mRNAs. Studies on Inflammatory Bowel Disease (IBD) in relation with miRNA are much less shown. The aim of the present study was to assess the expression patterns of microRNA 106a and microRNA 362-3p in peripheral blood samples of Inflammatory Bowel Disease (IBD) patients including Crohn's Disease(CD) and Ulcerative Colitis (UC).
Methods:
This study consisted of 32 CD, 32 UC patients and 32 controls. The expression level of the micro-RNAs -106a and -362-3p was determined using reverse transcription and real-time RT-PCR.
Results:
Our findings showed that MiR-106a and miR-362-3p are expressed at significantly higher levels in the peripheral blood from patients with CD and UC compared to controls. MiR-106a and miR-362-3p expression are also different in the peripheral blood of patients regarding the activity score of the disease. There were significant differences of miR362-3p in active UC relative to inactive UC.
Conclusion:
Altogether our findings suggest that miR-106a and miR-363-3p can play an important role in the pathogenesis of IBD. The differences in expression of miR106a and miR362-3p in peripheral blood of the UC and CD patients in an active phase in comparison to inactive disease suggest that these miRNAs may be useful as potential biomarkers for diagnosis and monitoring the disease activity.
Insights
MicroRNA-106a and microRNA-362-3p show elevated expression in Inflammatory Bowel Disease (IBD) patients. These microRNAs may serve as biomarkers for diagnosing and monitoring IBD activity.
Area of Science:
- Molecular Biology
- Genetics
- Immunology
Background:
- MicroRNAs (miRNAs) are key regulators of gene expression.
- Limited research exists on miRNA involvement in Inflammatory Bowel Disease (IBD).
Purpose of the Study:
- To investigate the expression patterns of microRNA-106a and microRNA-362-3p in the peripheral blood of IBD patients.
- To compare miRNA expression between Crohn's Disease (CD), Ulcerative Colitis (UC), and healthy controls.
Main Methods:
- Quantitative analysis of microRNA-106a and microRNA-362-3p expression.
- Utilized reverse transcription and real-time RT-PCR.
- Study included 32 CD patients, 32 UC patients, and 32 controls.
Main Results:
- Significantly higher expression of miR-106a and miR-362-3p was observed in IBD patients (CD and UC) compared to controls.
- Expression levels of these miRNAs correlated with disease activity.
- miR-362-3p showed distinct expression differences between active and inactive Ulcerative Colitis.
Conclusions:
- miR-106a and miR-362-3p may play a role in the pathogenesis of IBD.
- These miRNAs show potential as diagnostic and monitoring biomarkers for IBD activity.
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