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Updated: Feb 7, 2026

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer
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A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

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2-(2-nitrobenzylidene) indolin-3-one compound inhibits transmembrane prostate androgen-induced protein (TMEPAI)

Yuyin Li1, Jianjun Wang1, Ning Song1

  • 1Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.

Cell Proliferation
|August 3, 2018
PubMed
Abstract

Insights

A novel compound, JHY-A007-50, effectively inhibits the transmembrane prostate androgen-induced protein (TMEPAI) and suppresses cancer cell proliferation. This discovery offers a potential new therapeutic strategy for cancer treatment targeting TMEPAI.

Area of Science:

  • Oncology
  • Molecular Biology
  • Drug Discovery

Background:

  • Aberrant expression of transmembrane prostate androgen-induced protein (TMEPAI) is implicated in tumorigenesis.
  • TMEPAI represents a potential therapeutic target for cancer drug discovery.

Purpose of the Study:

  • To screen for compounds that inhibit TMEPAI expression.
  • To evaluate the effects of identified inhibitors on cancer cell proliferation.

Main Methods:

  • A firefly luciferase reporter assay was used to screen for TMEPAI inhibitors.
  • Cancer cell proliferation was assessed via colony formation, cell cycle analysis, Ki-67 immunofluorescence, and EdU incorporation assays.

Main Results:

  • 2-(2-nitrobenzylidene) indolin-3-one (JHY-A007-50) was identified as a potent inhibitor of TMEPAI promoter activity.
  • JHY-A007-50 suppressed TMEPAI expression at both mRNA and protein levels, inhibited cancer cell proliferation, and induced G1 phase cell cycle arrest.
  • Overexpression of TMEPAI reduced the inhibitory effects of JHY-A007-50, confirming TMEPAI's role in mediating the compound's action.

Conclusions:

  • Compound JHY-A007-50 effectively downregulates TMEPAI expression.
  • JHY-A007-50 demonstrates significant potential in inhibiting cancer cell proliferation, highlighting its therapeutic promise.

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