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Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

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Combining Affinity Selection and Specific Ion Mobility for Microchip Protein Sensing.

William E Arter^1,2, Jérôme Charmet^2,3, Jinglin Kong¹

¹Cavendish Laboratory , University of Cambridge , JJ Thomson Avenue , Cambridge CB3 0HE , U.K.

Analytical Chemistry

|August 3, 2018

Summary

This summary is machine-generated.

This study introduces a novel assay for sensitive protein detection in complex biological fluids. The method combines electrophoresis and affinity selection with DNA nanocircuits for high selectivity and signal amplification, overcoming limitations of traditional assays.

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Area of Science:

Biomolecular science
Biophysics
Diagnostics

Background:

Sensitive protein detection is crucial in biomolecular science.
Complex biological fluids cause nonspecific interactions, hindering accurate sensing.
Conventional assays like ELISA have limitations, especially for monoepitopic targets.

Purpose of the Study:

To develop a highly selective protein detection assay for complex biological fluids.
To overcome limitations of existing methods by combining chemical and physical properties for target selection.
To demonstrate signal amplification using a catalytic DNA nanocircuit.

Main Methods:

A combinatorial strategy using free-flow electrophoresis for electrophoretic mobility-based target selection.
Simultaneous application of conventional affinity-based selection.
Signal amplification via a catalytic DNA nanocircuit.
Microfluidic sample handling with isothermal, enzyme-free signal amplification.

Main Results:

Achieved high selectivity in complex media by exploiting both chemical and physical properties.
Demonstrated an assay requiring no surface immobilization or washing steps.
Successfully applied the assay to monoepitopic targets, showing advantages over ELISA.

Conclusions:

The developed assay offers a sensitive and selective method for protein detection in challenging biological samples.
This approach integrates microfluidics and DNA nanocircuits for efficient, enzyme-free signal amplification.
The assay presents a significant advancement over conventional techniques like ELISA, particularly for specific target detection.