Related Experiment Video
Updated: Feb 7, 2026
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
Published on: April 14, 2010
LncRNA H19 regulates the expression of its target gene HOXA10 in endometrial carcinoma through competing with miR-612
1Department of Obstetrics, Dezhou People's Hospital, Dezhou, China. yu.acool@163.com.
Objective:
The role of long non-coding RNA (lncRNA) H19 in endometrial carcinoma was studied, and its mechanism was also explored.
Patients And Methods:
Quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR) was used to detect the expression of lncRNA H19, miR-612, and HOXA10 in endometrial carcinoma, and the relationship between lncRNA H19 and survival time was analyzed. The high expression or knockdown of lncRNA H19 in endometrial cancer cells was completed by cell transfection experiments. Cell counting kit-8 (CCK-8) assay was used to detect changes in the viability of endometrial cancer cells. Dual luciferase reporter assay was performed to verify that miR-612 could bind to lncRNA H19 or HOXA10. QRT-PCR and Western Blot assays were used to detect changes in the expression of HOXA10 in endometrial cancer cells before and after overexpression or knockdown of lncRNA H19.
Results:
The expression of lncRNA H19 and HOXA10 was high in endometrial carcinoma and miR-612 was lowly expressed. The survival curve suggested that lncRNA H19 was negatively correlated with patient survival. The mRNA expression of lncRNA H19 in endometrial cancer cells, including HEC1-A, HEC1-B, AN3CA, and Ishikawa, was detected by qRT-PCR. It was found that the expression of lncRNA H19 was highest in AN3CA and lowest in Ishikawa. The cell transfection experiments allowed Ishikawa cells to overexpress lncRNA H19 and AN3CA cells to reduce lncRNA H19 expression. After overexpression of lncRNA H19, the viability of Ishikawa cells as well as the mRNA and protein levels of HOXA10 increased. However, after knocking down lncRNA H19, the viability of AN3CA cells along with the mRNA and protein levels of HOXA10 decreased. The dual luciferase reporter assay results suggested that miR-612 could bind to lncRNA H19 and HOXA10.
Conclusions:
The high expression of lncRNA H19 in endometrial carcinoma may regulate the expression level of its target gene HOXA10 by targeting miR-612, thus promoting cell proliferation to play a role in the development of endometrial carcinoma.
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
02:35Chromatin Position Affects Gene Expression
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
01:27Constitutive and Regulated Gene Expression
01:42What is Gene Expression?
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
01:36What is Gene Expression?
02:24Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...