Related Experiment Video
Updated: Feb 7, 2026

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
Published on: July 28, 2023
[The effects of down-regulated ITGB5 expression on the proliferation of keloid fibroblasts]
Objective:
To explore the effects of down-regulated ITGB5 expression on the proliferation of keloid fibroblasts and clarify the possible role of β5-integrin (ITGB5) in keloid.
Methods:
Construct lentiviral sh-RNA-expression vector targeting ITGB5 and infect keloid fibroblasts, the expression of ITGB5 were detected by Western Blot, the proliferation ability was identified by MTT.
Results:
The expression quantity of ITGB5 mRNA and protein in KFb group,LV-NC group and LV-KFb group are 1.00 ± 0.00,1.08 ±0.05,0.34 ±0.01 and 0.91 ±0.03,0.93 ±0.02,0.28 ±0.07.Compared with LV-NC group and KFb group, the expression quantity of ITGB5 mRNA and protein in LV-KFb group decreased significantly (P < 0.01).Compared with LV-NC group and KFb group,the proliferation rate decreased significantly in LV-KFb group at 48 h(P < 0.01).
Conclusions:
These results suggest that ITGB5 can accelerate fibroblasts proliferation in keloids.
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