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A high-throughput technique to quantify bacterial pathogens' virulence on the insect model Galleria mellonella
Nathalie Parthuisot1, Jacques Rouquette2, Jean-Baptiste Ferdy1
1Évolution et Diversité Biologique - UMR 5174 CNRS-UPS Université Paul Sabatier - F-31062, Toulouse cedex 9, France.
Journal of Microbiological Methods
|August 3, 2018
Abstract:
We combined spectrophotometry and an original statistical approach to infer bacteria virulence, using the lepidoptera Galleria mellonella as a host model. With this method, it is possible to use a microplate reader to automatize data collection on host survival on batches of 96 samples. The method also allows measurement of pathogen multiplication if GFP labelled bacterial strains are used.