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Related Concept Videos

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The parallel RLC circuit is an arrangement where the resistor (R), inductor (L), and capacitor (C) are all connected to the same nodes and, as a result, share the same voltage across them. The parallel RLC circuit is analyzed in terms of admittance (Y), which reflects the ease with which current can flow. The admittance is given by:
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The brain processes sensory information rapidly due to parallel processing, which involves sending data across multiple neural pathways at the same time. This method allows the brain to manage various sensory qualities, such as shapes, colors, movements, and locations, all concurrently. For instance, when observing a forest landscape, the brain simultaneously processes the movement of leaves, the shapes of trees, the depth between them, and the various shades of green. This enables a quick and...
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Resistors are in parallel when one end of all the resistors are connected to a continuous wire of negligible resistance and the other end of all the resistors are also connected to one another through a continuous wire of negligible resistance. In the case of a parallel configuration, the potential drop across each resistor is the same. Current through each resistor can be found using Ohm’s law, I = V/R, where the voltage is constant across each resistor. The sum of the individual currents...
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Multiple capacitors connected serve as electrical components in various applications. These multiple capacitors behave as a single equivalent capacitor, and its total capacitance depends on the capacitance of individual capacitors and the type of connections. Capacitors can be arranged in two - orientations, either in series or parallel connections.
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Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
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toaSTR: A web application for forensic STR genotyping by massively parallel sequencing.

Sebastian Ganschow1, Janine Silvery1, Jörn Kalinowski2

  • 1LABCON-OWL Analytik, Forschung und Consulting GmbH, Siemensstr. 40, 32105 Bad Salzuflen, Germany.

Forensic Science International. Genetics
|August 3, 2018
PubMed
Summary

Massively parallel sequencing (MPS) offers high-resolution DNA analysis for forensic short tandem repeat (STR) genotyping. The toaSTR software simplifies complex MPS data interpretation for forensic experts, improving casework efficiency.

Keywords:
DNA typingMassively parallel sequencing (MPS)Next-generation sequencing (NGS)Short tandem repeat (STR)Web application

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Area of Science:

  • Forensic genetics
  • Bioinformatics
  • Molecular biology

Background:

  • Massively parallel sequencing (MPS) is a powerful technique for high-resolution short tandem repeat (STR) genotyping in forensics.
  • The complexity of MPS data presents challenges for routine casework analysis.
  • Efficient interpretation tools are needed to leverage MPS technology in forensic science.

Purpose of the Study:

  • To develop a user-friendly web application, toaSTR, for simplifying and enhancing the analysis of MPS data in forensic STR genotyping.
  • To provide forensic DNA analysts with a tool that efficiently handles complex sequence data generated by MPS platforms.
  • To assist in the accurate differentiation of biological alleles from artefacts in forensic samples.

Main Methods:

  • Development of a web application with a graphical user interface for STR genotyping workflow.
  • Implementation of a sequence-based stutter model to predict and identify stutter variants.
  • Integration of data visualization tools and an interface for biostatistics and reporting.
  • Compatibility and concordance studies using diverse MPS platforms, PCR kits, and chemistries.

Main Results:

  • toaSTR successfully handles data from various MPS platforms and multiplex PCR kits.
  • The software accurately differentiates biological alleles from stutter and artefacts, aiding mixed sample interpretation.
  • Compatibility and concordance studies confirmed toaSTR's independent functionality and precise allele calling.
  • The application offers comprehensive data visualization and compliant PDF reporting.

Conclusions:

  • toaSTR provides an efficient and accessible solution for forensic experts analyzing MPS data for STR genotyping.
  • The software's features, including its stutter model and visualization tools, enhance the interpretation of complex forensic DNA profiles.
  • toaSTR facilitates the routine application of MPS in forensic casework, supporting accurate and reliable DNA analysis.