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Identification of Circular RNAs using RNA Sequencing
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Circular RNA ciRS-7 Maintains Metastatic Phenotypes as a ceRNA of miR-1299 to Target MMPs

Meixiang Sang1, Lingjiao Meng1, Sihua Liu1

  • 1Research Center and Tumor Research Institute, the Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

Molecular Cancer Research : MCR
|August 4, 2018
PubMed

Insights

Circular RNA ciRS-7 is highly expressed in triple-negative breast cancer (TNBC), promoting cell migration and invasion. Downregulating ciRS-7 inhibits TNBC metastasis by affecting the miR-1299/MMP pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNA ciRS-7 is known to regulate miR-7.
  • The role of ciRS-7 in triple-negative breast cancer (TNBC) is not well understood.
  • TNBC is an aggressive breast cancer subtype with limited treatment options.

Purpose of the Study:

  • To investigate the role of ciRS-7 in TNBC.
  • To determine if ciRS-7 regulates other miRNAs in TNBC.
  • To elucidate the mechanism by which ciRS-7 influences TNBC progression.

Main Methods:

  • Quantitative real-time PCR to measure ciRS-7 expression in TNBC tissues and cells.
  • Cell migration and invasion assays (in vitro).
  • In vivo metastasis assays in mice.
  • MiRNA binding site analysis and luciferase reporter assays to confirm ciRS-7/miR-1299 interaction.
  • Western blotting to assess matrix metalloproteinase (MMP) expression.

Main Results:

  • ciRS-7 expression is significantly upregulated in TNBC specimens and cells compared to other breast cancer subtypes.
  • Downregulation of ciRS-7 inhibited TNBC cell migration, invasion, and metastasis in vivo.
  • ciRS-7 acts as a competing endogenous RNA (ceRNA) for miR-1299 in TNBC cells.
  • ciRS-7 enhances the expression of matrix metalloproteinases (MMPs) by sponging miR-1299, thereby promoting TNBC cell migration and invasion.

Conclusions:

  • Circular RNA ciRS-7 is a key regulator of TNBC progression.
  • ciRS-7 promotes TNBC cell migration, invasion, and metastasis through the miR-1299/MMP pathway.
  • ciRS-7 represents a potential therapeutic target for TNBC.

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