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Updated: Feb 7, 2026

Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells
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Cigarette smoke upregulates SPRR3 by favoring c-Jun/Fra1 heterodimerization in human bronchial epithelial cells
Xiwei Hu1,2, Nianchun Peng3, Fei Qi2
1Department of Respiratory Medicine, The Second Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, PR China.
Aim:
The airway epithelium of smokers exhibits upregulated SPRR3, an indicator of pathogenic keratinization. The mechanisms underlying this phenomenon require investigation.
Patients & Methods:
Human bronchial epithelial (HBE) SPRR3 expression was analyzed by smoking status. Primary HBE cells were exposed to cigarette smoke (CS). SPRR3 expression, SPRR3 promoter activity, AP-1 factor binding and AP-1 factors' effects were analyzed.
Results:
Current smokers display SPRR3 upregulation relative to never smokers. CS upregulates SPRR3 transcription in an exposure-dependent manner. CS promotes c-Jun and Fra1 binding to the SPRR3-AP-1/TRE site. Wild-type c-Jun and Fra1 upregulate, whereas c-Jun and Fra1, dominant-negative mutants, suppress SPRR3 promoter activity.
Conclusion:
CS induces SPRR3 upregulation in HBE cells by promoting aberrant c-Jun/Fra1 dimerization.
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