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Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions
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Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions

Published on: August 8, 2012

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Conditioned Medium from Human Umbilical Vein Endothelial Cells Promotes Proliferation, Migration, Invasion and

Ming-Lian Luo1,2, Xiao-Ping Liu1, Fang Wang1

  • 1Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Current Medical Science
|August 4, 2018
PubMed

Insights

Induced endothelial differentiation of adipose-derived stem cells (ADSCs) shows potential for treating preeclampsia (PE). These modified ADSCs enhanced vascular repair factors, suggesting a novel therapeutic approach for this pregnancy complication.

Area of Science:

  • Regenerative Medicine
  • Obstetrics
  • Vascular Biology

Background:

  • Preeclampsia (PE) is a pregnancy complication linked to endothelial dysfunction.
  • Adipose-derived stem cells (ADSCs) can differentiate into endothelial cells, offering potential for vascular repair.

Purpose of the Study:

  • To investigate the potential of induced endothelial differentiation of ADSCs (iADSCs) for treating PE.
  • To compare the bioactivity and angiogenic factor expression of iADSCs versus standard ADSCs.

Main Methods:

  • ADSCs and human umbilical vein endothelial cells (HUVECs) were isolated.
  • ADSCs were induced to differentiate using HUVEC-conditioned medium.
  • Flow cytometry, proliferation, migration, invasion, angiogenesis assays, RT-PCR, and Western blotting were used to assess cell characteristics and function.

Main Results:

  • HUVEC-conditioned medium promoted ADSC proliferation, migration, invasion, and angiogenesis.
  • Induced ADSCs (iADSCs) exhibited enhanced endothelial characteristics.
  • Levels of vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF) were significantly increased in iADSCs.

Conclusions:

  • Induced endothelial differentiation of ADSCs holds promise for PE therapy.
  • iADSCs demonstrate enhanced angiogenic potential relevant for treating PE-related vascular dysfunction.

Keywords:
adipose derived stem cellsangiogenesisendothelial differentiationpreeclampsia

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