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Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles and a Simple Method for Dane Particle Enrichment
Takanori Wagatsuma1,2, Atsushi Kuno1, Kiyohiko Angata1
1Research Center for Medical Glycoscience (RCMG) , National Institute of Advanced Industrial Science and Technology , AIST Tsukuba Central 2, 1-1-1, Umezono , Tsukuba , Ibaraki 305-8568 , Japan.
Analytical Chemistry
|August 4, 2018
Summary
This study introduces a new lectin microarray to profile Hepatitis B virus (HBV) surface antigen (HBsAg) glycans. Jacalin lectin fractionation enriches infectious Dane particles from patient serum, aiding HBV research.
Area of Science:
- Virology
- Glycobiology
- Immunology
Background:
- Hepatitis B virus (HBV) exists as infectious Dane particles and abundant noninfectious subviral particles (SVPs).
- Dane particles, covered by HBV surface antigen (HBsAg), are the infectious form.
- HBsAg glycosylation influences viral particle formation and secretion.
Purpose of the Study:
- To develop a sensitive method for profiling HBsAg glycans on intact HBV particles.
- To establish a method for enriching infectious Dane particles from patient serum.
Main Methods:
- A novel lectin microarray system was used for sensitive glycan profiling of HBsAg in patient serum.
- Jacalin, an O-glycan-recognizing lectin, showed specific reactivity to M-HBsAg.
- Lectin-fractionation using jacalin separated HBV particles into bound and unbound fractions.
Main Results:
- The lectin microarray successfully profiled glycans on intact HBV particles.
- Jacalin demonstrated strong reactivity to M-HBsAg, crucial for Dane particle secretion.
- The jacalin-bound fraction contained a significant portion of the HBV viral DNA load, indicating enrichment of Dane particles.
Conclusions:
- A novel glycan profiling method for HBsAg on intact HBV particles was established.
- Jacalin fractionation provides a simple and effective method to enrich infectious Dane particles from patient serum.
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