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Simple and inexpensive long-term preservation methods for Phytophthora infestans
Haichen Cui1, Xingbo Ren1, Lisha Yun1
1Department of Biochemistry and Molecular Biology, College of Life Sciences, Inner Mongolia Agricultural University (South campus of Inner Mongolia Agricultural University), Erdos Street, Saihan District, Hohhot 010010, Inner Mongolia, China.
Journal of Microbiological Methods
|August 4, 2018
Summary
Preserving Phytophthora infestans, the potato late blight pathogen, is crucial. Modified rye seed methods best maintain viability and prevent contamination, though optimal preservation varies by isolate for virulence.
Area of Science:
- Plant Pathology
- Microbial Preservation
Background:
- Phytophthora infestans causes devastating potato late blight.
- Long-term preservation is vital for research and fungicide development.
Purpose of the Study:
- To evaluate four preservation methods for Phytophthora infestans.
- To determine the best method for maintaining viability, growth, and virulence.
Main Methods:
- Compared mineral oil, sterile water, rye seed, and modified rye seed methods.
- Assessed survival, growth, morphology, and virulence of four P. infestans isolates.
Main Results:
- All methods maintained high viability; rye seed methods ensured 100% viability without contamination.
- Preservation methods did not significantly alter growth or morphology.
- Impact on virulence was isolate-specific, with different methods optimal for different isolates.
Conclusions:
- Modified rye seed methods are recommended for general P. infestans preservation.
- Utilizing multiple storage methods ensures isolate stability and safety.
- Tailoring preservation to specific isolates is key for maintaining virulence.
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