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The role of miR-29c/B7-H3 axis in children with allergic asthma

Xinxing Zhang1, Xin Zhao2, Huiming Sun1

  • 1Department of Respiratory Disease, Children's Hospital of Soochow University, Soochow University, Suzhou, China.

Journal of Translational Medicine
|August 5, 2018
PubMed
Abstract

Insights

MicroRNA-29c (miR-29c) is reduced in children with asthma exacerbation. Lower miR-29c and higher B7-H3 levels are linked to asthma, suggesting a new therapeutic target for allergic asthma.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs are implicated in bronchial asthma pathogenesis.
  • The specific role of miR-29c in allergic asthma remains largely unknown.
  • Understanding miR-29c's regulation of T-helper cell differentiation in macrophages is crucial.

Purpose of the Study:

  • To elucidate the regulatory role of miR-29c in T-helper cell differentiation within mononuclear macrophages.
  • To investigate the miR-29c/B7-H3 axis in pediatric asthma.

Main Methods:

  • Isolated CD14+ monocytes from children with asthma exacerbation and controls.
  • Utilized microarray analysis, qRT-PCR, and ELISA to assess miRNA and protein expression.
  • Performed transfection studies and luciferase reporter assays to confirm miR-29c targets and function.

Main Results:

  • Asthma patients exhibited significantly lower miR-29c and higher plasma B7-H3 levels compared to controls.
  • B7-H3 was identified as a direct target of miR-29c.
  • Inhibition of miR-29c enhanced Th2/Th17 cell differentiation markers (ROR-γt, GATA-3) and cytokine production (IL-4, IL-17).

Conclusions:

  • The miR-29c/B7-H3 axis plays a significant role in pediatric asthma.
  • This axis regulates Th2 and Th17 cell differentiation.
  • The miR-29c/B7-H3 pathway presents potential therapeutic targets for asthma treatment.
Keywords:
AsthmaB7-H3ChildrenMircoRNATh cell differentiation

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