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Updated: Feb 7, 2026

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
Published on: July 10, 2019
A one-step real-time RT-PCR helps to identify mixed rotavirus infections in Mexico
Yazmin Anaya-Molina1, Sergio Isaac De La Cruz Hernández2, Atenea Estela Andrés-Dionicio2
1Laboratorio de Virus Gastrointestinales, Departamento de Virología, Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE), Francisco de P Miranda 177, Lomas de Plateros, 01480, Álvaro Obregón, CDMX, Mexico; Laboratorio de Bacteriología Médica, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Santo Tomás, 11340, Miguel Hidalgo, CDMX, Mexico.
Abstract:
Rotaviruses continue being the most important pathogens responsible of diarrhea in young children worldwide. Seminested reverse transcription polymerase chain reaction (RT-PCR) is used to determine rotavirus genotype; however, this technique employs multistep procedures. The real-time RT-PCR is a fast and reliable tool that can be used as rotavirus genotyping tool, especially in rotavirus outbreaks. In this study, we tested a real-time RT-PCR to identify rotavirus genotype using a panel of 252 samples from patients with diarrheal disease caused by G9P[4] and G12P[8] genotypes, which were identified as emerging rotaviruses in 2 outbreaks in Chiapas, Mexico. Our results show that the real-time RT-PCR assay detected these rotaviruses, and it allowed us to identify mixed genotype infections, G/P combinations, and the viral abundance in some samples in which the seminested assay could not identify them. Therefore, the real-time RT-PCR is a molecular tool that can be great support during rotavirus outbreaks.
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