Plant organelle dynamics: cytoskeletal control and membrane contact sites

Contents Summary 381 I. Introduction 381 II. Basic movement characteristics 382 III. Actin and associated motors, myosins, play a primary role in plant organelle movement and positioning 382 IV. Mechanisms of myosin recruitment: a tightly regulated system? 384 V. Microtubules, associated motors and interplay with actin 386 VI. Role of organelle interactions: tales of tethers 387 VII. Summary model to describe organelle movement in higher plants 390 VIII. Why is organelle movement important? 390 IX. Conclusions and future perspectives 391 Acknowledgements 391 References 391 SUMMARY: Organelle movement and positioning are correlated with plant growth and development. Movement characteristics are seemingly erratic yet respond to external stimuli including pathogens and light. Given these clear correlations, we still do not understand the specific roles that movement plays in these processes. There are few exceptions including organelle inheritance during cell division and photorelocation of chloroplasts to prevent photodamage. The molecular and biophysical components that drive movement can be broken down into cytoskeletal components, motor proteins and tethers, which allow organelles to physically interact with one another. Our understanding of these components and concepts has exploded over the past decade, with recent technological advances allowing an even more in-depth profiling. Here, we provide an overview of the cytoskeletal and tethering components and discuss the mechanisms behind organelle movement in higher plants.