Related Experiment Video

Updated: Feb 7, 2026

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells
09:47

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Published on: December 9, 2022

4.6K

Cell specific microvesicles vary with season and disease predisposition in healthy and previously laminitic ponies

E J T Finding1, C Lawson2, J Elliott2

  • 1Department of Clinical Sciences and Services, Royal Veterinary College, Hatfield, AL9 7TA, UK.

Veterinary Immunology and Immunopathology
|August 7, 2018
PubMed

Insights

Researchers identified equine cell-specific microvesicles in pony plasma, revealing seasonal variations and differences in endothelial microvesicles between healthy and laminitic horses. This opens new avenues for equine health research.

Area of Science:

  • Veterinary Medicine
  • Cell Biology
  • Immunology

Background:

  • Microvesicles are key intercellular communicators found in bodily fluids.
  • Altered microvesicle levels serve as disease biomarkers.
  • Equine microvesicle research, particularly concerning laminitis, is limited.

Purpose of the Study:

  • To identify and quantify equine cell-specific microvesicles in pony plasma.
  • To investigate microvesicle profiles in healthy versus laminitic ponies.
  • To establish a foundation for studying microvesicles in equine health and disease.

Main Methods:

  • Developed a cross-reactive antibody panel for flow cytometry.
  • Detected microvesicles of platelet, leukocyte, and endothelial origin.
  • Analyzed plasma from healthy and laminitic ponies.

Main Results:

  • Quantified total microvesicles and cell-specific proportions.
  • Observed seasonal variations in microvesicle numbers and types.
  • Found higher annexin V-positive endothelial microvesicles in non-laminitic ponies compared to previously laminitic ones.

Conclusions:

  • Established a novel method for equine microvesicle analysis.
  • Demonstrated seasonal and disease-related differences in equine microvesicles.
  • Highlighted the potential of microvesicles as biomarkers in equine laminitis and other diseases.
Keywords:
EquineFlow cytometryLaminitisMicrovesicle

More Related Videos

Isolation and Characterization of Microvesicles from Peripheral Blood06:03

Isolation and Characterization of Microvesicles from Peripheral Blood

Published on: January 6, 2017

23.8K
Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes05:26

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Published on: April 29, 2020

6.7K

Related Concept Videos

Seasoning of Wood01:15

Seasoning of Wood

Seasoning of wood is a crucial process aimed at reducing and stabilizing the moisture content within the wood to prevent future shrinkage, structural damage, or aesthetic issues once the wood is used in construction. Wood naturally swells when it absorbs moisture and contracts as it dries.
Achieving equilibrium moisture content is the goal of seasoning; this is the point where the wood's moisture content stabilizes to align with the moisture levels of the surrounding environment. Proper...
519
Biological Clocks and Seasonal Responses02:45

Biological Clocks and Seasonal Responses

The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
41.7K
Gradually Varying Flow01:29

Gradually Varying Flow

Gradually varying flow (GVF) in open channels describes situations where water depth changes slowly along the channel due to factors like non-uniform bed slope, channel shape variations, or obstructions. This flow type occurs when the depth adjusts gradually to balance gravitational forces, shear forces, and energy requirements, resulting in a low rate of depth change.Characteristics of Gradually Varying FlowGVF is commonly observed in natural streams, rivers, and canals, where flow depth...
445
Rapidly Varying Flow01:24

Rapidly Varying Flow

Rapidly varying flow (RVF) in open channels is characterized by abrupt changes in flow depth over a short distance, with the rate of depth change relative to distance often approaching unity. These flows are inherently complex due to their transient and multi-dimensional nature, making exact analysis difficult. However, approximate solutions using simplified models provide valuable insights into their behavior.Key Features of Rapidly Varying FlowRVF is commonly observed in scenarios involving...
505
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.6K