Related Experiment Video

Updated: Feb 7, 2026

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria
11:32

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

Published on: February 23, 2014

15.7K

A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties

Xinyu Miao1, Jiaojiao He1, Liang Zhang1

  • 1Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou, China.

Frontiers in Microbiology
|August 7, 2018
PubMed

Insights

Streptococcus pneumoniae utilizes SPD_1590, a novel iron transporter, to acquire essential iron, likely supplementing the PiuABC system. This transporter is crucial for bacterial virulence, enhancing our understanding of this pathogen.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Pathogen Research

Background:

  • Streptococcus pneumoniae employs PiaABC, PiuABC, and PitABC for iron acquisition.
  • Previous findings indicated SPD_1590 upregulation in iron transporter mutants.

Purpose of the Study:

  • To investigate the role of SPD_1590 as a novel iron transporter in Streptococcus pneumoniae.
  • To determine the contribution of SPD_1590 to bacterial virulence.

Main Methods:

  • Construction and analysis of spd1590-knockout, -complemented, and -overexpressing strains.
  • Purification and hemin-binding assays of SPD_1590 protein.
  • iTRAQ quantitative proteomics and cell-infection studies.

Main Results:

  • SPD_1590 directly binds hemin, suggesting a role in iron acquisition, potentially complementing PiuABC.
  • SPD_1590 is essential for virulence properties of Streptococcus pneumoniae.
  • Proteomics data revealed SPD_1590's involvement in metal-ion uptake pathways.

Conclusions:

  • SPD_1590 is identified as a novel hemin-binding iron transporter in Streptococcus pneumoniae.
  • SPD_1590 plays a significant role in the virulence of this important human pathogen.
Keywords:
S. pneumoniaebacterial virulencehemin bindingiron transporterproteomics

More Related Videos

Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction04:25

Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction

Published on: February 24, 2014

46.6K
Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae09:12

Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae

Published on: January 17, 2014

13.2K

Related Concept Videos

Electron Transport Chains01:28

Electron Transport Chains

The final stage of cellular respiration is oxidative phosphorylation that consists of two steps: the electron transport chain and chemiosmosis. The electron transport chain is a set of proteins found in the inner mitochondrial membrane in eukaryotic cells. Its primary function is to establish a proton gradient that can be used during chemiosmosis to produce ATP and generate electron carriers, such as NAD+ and FAD, that are used in glycolysis and the citric acid cycle.
The ETC is comprised of...
112.4K
Pneumonia I: Introduction01:30

Pneumonia I: Introduction

Pneumonia is an acute respiratory infection that targets the lungs, specifically the alveoli. These tiny air sacs, essential for oxygen exchange, become engorged with pus and fluid, severely hindering breathing, decreasing oxygen absorption, and causing significant pain and discomfort during respiration.
Risk Factors
Various factors influence the likelihood of developing pneumonia. Age plays a crucial role, with infants, children under two, and individuals over 65 at increased risk due to their...
903
Pneumonia II: Pathophysiology01:29

Pneumonia II: Pathophysiology

The pathophysiology of pneumonia involves the following steps:
3.0K
Binet's Contribution to Measures of Intelligence01:23

Binet's Contribution to Measures of Intelligence

Alfred Binet, along with his student Théophile Simon, was tasked by the French Ministry of Education in 1904 to create a method for identifying students who struggled to learn through conventional classroom instruction. This initiative aimed to address overcrowding by placing such students in specialized schools. Binet and Simon developed an intelligence test comprising 30 tasks, ranging from simple commands, like touching one's nose or ear, to more complex tasks, such as drawing...
1.7K
General Properties of Solutions02:12

General Properties of Solutions

Many common substances around us exist as a solution, such as ocean water, air, and gasoline. All solutions are mixtures of substances that are composed of varying amounts of two or more types of atoms or molecules. A mixture with a non-uniform composition is a heterogeneous mixture, whereas a mixture with a uniform composition is a homogeneous mixture. The components that make the homogeneous mixture are evenly spread out and thoroughly mixed. 
36.0K
Facilitated Transport01:19

Facilitated Transport

The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In  facilitated transport, also known as facilitated diffusion, molecules and ions travel across a...
149.3K