Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

14.0K
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
14.0K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

12.5K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
12.5K
Primary Active Transport01:47

Primary Active Transport

199.4K
In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps that are embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction...
199.4K
Anatomy of Chloroplasts01:07

Anatomy of Chloroplasts

119.4K
Green algae and plants, including green stems and unripe fruit, harbor chloroplasts—the vital organelles where photosynthesis takes place. In plants, the highest density of chloroplasts is found in the mesophyll cells of leaves.
119.4K
Chemiosmosis01:32

Chemiosmosis

114.6K
Oxidative phosphorylation is a highly efficient process that generates large amounts of adenosine triphosphate (ATP), the basic unit of energy that drives many cellular processes. Oxidative phosphorylation involves two processes— the electron transport chain and chemiosmosis.
Electron Transport Chain
The electron transport chain involves a series of protein complexes on the inner mitochondrial membrane that undergo a series of redox reactions. At the end of this chain, the electrons...
114.6K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

8.8K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A high-resolution functional network-organized atlas of human superficial white matter from ultra-high-field diffusion MRI.

iScience·2026
Same author

Chimeric switch scaffold protein augments CAR synapse formation and signaling networks.

Journal for immunotherapy of cancer·2026
Same author

Vitamin/mineral and non-vitamin/non-mineral supplement use of breast cancer survivors in Korea.

Nutrition research and practice·2026
Same author

Biologically inspired microlens array camera for high-resolution wide field-of-view imaging.

Nature communications·2026
Same author

Long-term associations between perinatal factors and white matter microstructure at 8-10 years.

Frontiers in human neuroscience·2026
Same author

Ultrasensitive Detection of Multiple Foodborne Pathogens Using CRISPR-Cas12a on a Finger-Actuated Microfluidic Device Integrated with a Modular Pressurizing Pump.

Analytical chemistry·2025

Related Experiment Video

Updated: Feb 7, 2026

Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls
06:43

Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls

Published on: July 27, 2022

9.6K

Microfluidic on-chip immunohistochemistry directly from a paraffin-embedded section.

Chang Hyun Cho1, Seyong Kwon1, Segi Kim1

  • 1Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

Biomicrofluidics
|August 7, 2018
PubMed
Summary

A novel microfluidic platform enables on-chip immunohistochemistry (IHC) on tissue slides. This PUA chip streamlines IHC processes, reducing time by over 50% with comparable results to conventional methods.

More Related Videos

Author Spotlight: Enhancing Multicolor Fluorescence Localization in Lung Carcinoma Sample
05:00

Author Spotlight: Enhancing Multicolor Fluorescence Localization in Lung Carcinoma Sample

Published on: November 21, 2023

2.8K
Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
06:32

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Published on: January 9, 2019

8.3K

Related Experiment Videos

Last Updated: Feb 7, 2026

Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls
06:43

Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls

Published on: July 27, 2022

9.6K
Author Spotlight: Enhancing Multicolor Fluorescence Localization in Lung Carcinoma Sample
05:00

Author Spotlight: Enhancing Multicolor Fluorescence Localization in Lung Carcinoma Sample

Published on: November 21, 2023

2.8K
Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
06:32

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Published on: January 9, 2019

8.3K

Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Pathology

Background:

  • Immunohistochemistry (IHC) is crucial for disease diagnosis, but conventional methods are time-consuming and labor-intensive.
  • Existing microfluidic IHC platforms have limitations in consecutive processing and material compatibility.
  • Formalin-fixed paraffin-embedded (FFPE) tissues are standard for pathological analysis.

Purpose of the Study:

  • To develop and validate a novel microfluidic platform for performing complete immunohistochemistry (IHC) processes on-chip.
  • To evaluate the performance of the on-chip IHC compared to conventional methods.
  • To demonstrate the platform's utility for specific diagnostic applications, such as HER2 classification in breast cancer.

Main Methods:

  • Fabrication of a microfluidic chip using organic solvent-resistant polyurethane acrylate (PUA).
  • Development of a pressure-based reversible assembly method for sealing the chip to FFPE tissue slides.
  • Consecutive on-chip execution of deparaffinization, antigen retrieval, and immunoreaction steps.
  • Comparison of immunostaining intensity and diagnostic accuracy between on-chip and conventional IHC.

Main Results:

  • The PUA microfluidic chip successfully performed consecutive on-chip IHC processes.
  • On-chip IHC achieved results equivalent to conventional methods in terms of immunostaining intensity.
  • The total process time for on-chip IHC was reduced by more than 50%.
  • Accurate HER2 classification in breast cancer tissues was demonstrated using the on-chip platform.

Conclusions:

  • The novel microfluidic platform offers an efficient and effective alternative for on-chip immunohistochemistry.
  • The PUA chip facilitates streamlined, automated, and precise IHC, significantly reducing processing time.
  • This technology holds promise for developing advanced diagnostic devices, including point-of-care applications.