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Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.2K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.4K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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Capillary Beds01:20

Capillary Beds

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Capillary beds are networks of tiny blood vessels that play a crucial role in the circulatory system. These beds are where the exchange of gases, nutrients, and waste products occurs between the blood and surrounding tissues. Each capillary bed consists of numerous capillaries, which are the smallest blood vessels in the body, typically only one cell-thick. This thinness allows for the efficient diffusion of substances.
Capillaries connect arterioles, small branches of arteries, to venules,...
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Capillary Exchange01:28

Capillary Exchange

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The cardiovascular system's chief role is to disseminate gases, nutrients, waste, and other substances to the body's cells. Small molecules like gases, lipids, and lipid-soluble substances directly diffuse through capillary wall endothelial cell membranes. Glucose, amino acids, and ions, including sodium, potassium, calcium, and chloride, use transporters for facilitated diffusion via membrane-specific channels. Glucose, ions, and bigger molecules may also pass through intercellular...
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Electrophoresis: Overview01:20

Electrophoresis: Overview

4.1K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
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Surface Tension, Capillary Action, and Viscosity02:57

Surface Tension, Capillary Action, and Viscosity

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Surface Tension
The various IMFs between identical molecules of a substance are examples of cohesive forces. The molecules within a liquid are surrounded by other molecules and are attracted equally in all directions by the cohesive forces within the liquid. However, the molecules on the surface of a liquid are attracted only by about one-half as many molecules. Because of the unbalanced molecular attractions on the surface molecules, liquids contract to form a shape that minimizes the number...
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Related Experiment Video

Updated: Feb 7, 2026

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
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Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

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Laser-Induced Fluorometry for Capillary Electrophoresis.

Takashi Kaneta1

  • 1Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushimanaka, Okayama, 700-8530, Japan.

Chemical Record (New York, N.Y.)
|August 7, 2018
PubMed
Summary

Capillary electrophoresis coupled with laser-induced fluorescence (CE-LIF) offers unparalleled sensitivity and resolution for detecting molecules. This review details CE-LIF methods and their applications in bioanalysis, including DNA, proteins, and drug determination.

Keywords:
DNA sequencingcapillary electrophoresislaser-induced fluorescencemicellar electrokinetic chromatography

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Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
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Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis

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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

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Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

Background:

  • Laser-induced fluorometry (LIF) provides single-molecule detection sensitivity.
  • Capillary electrophoresis (CE) offers high-resolution separation capabilities.
  • Combining LIF and CE creates a powerful analytical technique.

Purpose of the Study:

  • To review CE-LIF methods developed by the author's group.
  • To highlight applications in bioanalytical chemistry.
  • To showcase advancements in sensitive and high-resolution analysis.

Main Methods:

  • Indirect and direct detection using diode lasers.
  • Post-column derivatization techniques.
  • Hadamard transformation for enhanced data analysis.

Main Results:

  • CE-LIF demonstrates excellent performance in separating and detecting DNAs, proteins, and small molecules.
  • The technique is applicable to DNA binding assays, protein immunoassays, and anticancer drug determination.
  • Developed methods offer high sensitivity and resolution for complex biological samples.

Conclusions:

  • CE-LIF is an unrivaled analytical technique for sensitivity and resolution in bioanalysis.
  • The reviewed methods provide versatile tools for various biochemical and pharmaceutical analyses.
  • Further applications of CE-LIF in scientific research are anticipated.