Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Slow bleach-induced birefringence changes in rod outer segments.

M W Kaplan, P A Liebman

    The Journal of Physiology
    |March 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Radiance of deuterium arc lamps.

    Applied optics·2010
    Same author

    Multiple steps of phosphorylation of activated rhodopsin can account for the reproducibility of vertebrate rod single-photon responses.

    The Journal of general physiology·2003
    Same author

    Rapid irreversible G protein alpha subunit misfolding due to intramolecular kinetic bottleneck that precedes Mg2+ "lock" after GTP/GDP exchange.

    Biochemistry·2001
    Same author

    Predictability of weak binding from X-ray crystallography: inhaled anesthetics and myoglobin.

    Biochemistry·2001
    Same author

    Cooperative binding of inhaled anesthetics and ATP to firefly luciferase.

    Proteins·2001
    Same author

    Halothane binding to a G protein coupled receptor in retinal membranes by photoaffinity labeling.

    Biochemistry·2000
    Same journal

    Who are you, ketamine? Good, evil, or dose- and context-dependent?

    The Journal of physiology·2026
    Same journal

    Nuances in explaining the blunted erythropoietic response at altitude following recombinant human erythropoietin treatment at sea level.

    The Journal of physiology·2026
    Same journal

    Sex-dependent responses to glucagon agonist therapies in obesity: Mechanistic insights and broader pharmacological implications.

    The Journal of physiology·2026
    Same journal

    Brain sparing in fetal growth restriction: The double-edged sword of fetal hypoxaemia.

    The Journal of physiology·2026
    Same journal

    Protein kinase Cδ and pharmacomechanical coupling: Re-envisioning cerebral vascular control.

    The Journal of physiology·2026
    Same journal

    Improved subjective sleep quality in older adults by enhancing the GABAergic system in the sensorimotor cortex.

    The Journal of physiology·2026
    See all related articles

    Complex structural changes in rod outer segments (ROS) after a bleaching flash depend on membrane integrity. Intact ROS show biphasic birefringence changes linked to metarhodopsin II and retinol formation, unlike ruptured ROS.

    Area of Science:

    • Biophysics
    • Photochemistry
    • Cell Biology

    Background:

    • Rod outer segments (ROS) are photoreceptor cells responsible for vision.
    • Birefringence measurements are used to study structural dynamics in biological systems.
    • Rhodopsin photolysis triggers a cascade of molecular events within ROS.

    Purpose of the Study:

    • To investigate the time-resolved structural changes in ROS following a bleaching flash.
    • To determine the role of the outer segment envelope membrane's integrity in these structural dynamics.
    • To elucidate the influence of ionic environment and glycerol on phototransduction.

    Main Methods:

    • Time-resolved birefringence measurements were performed on ROS.
    • ROS envelope membrane integrity was manipulated (intact vs. ruptured).

    Related Experiment Videos

  • Ionic composition of the bathing medium (Na+ vs. K+) and glycerol concentration were varied.

    • Main Results:

    • Intact ROS exhibited a biphasic birefringence loss correlated with metarhodopsin II and retinol formation, followed by recovery.
    • Ruptured ROS showed initial losses but lacked full recovery and retinol formation.
    • Ionic environment and glycerol significantly altered the kinetics and magnitude of metarhodopsin formation and birefringence changes.

    Conclusions:

    • ROS structural dynamics post-bleaching are critically dependent on envelope membrane integrity.
    • Metarhodopsin II and retinol formation are key events influencing birefringence in intact ROS.
    • Environmental factors like ion composition and glycerol modulate phototransduction pathways.