Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Urinary System01:13

Introduction to Urinary System

9.1K
The urinary system consists of two kidneys, two ureters, the urinary bladder, and the urethra.
The kidneys are bean-shaped organs located in the retroperitoneal space, on either side of the vertebral column, between the T12 and L3 vertebrae. They are partially protected by the rib cage and surrounded by perirenal fat, which provides cushioning. They are responsible for urine formation and play critical roles in regulating blood pressure, electrolyte levels, and hormone production. The ureters...
9.1K
Urinary Bladder01:23

Urinary Bladder

3.5K
The urinary bladder is a hollow, muscular sac that temporarily stores urine before it is expelled from the body. It can hold approximately 600 mL of urine prior to micturition. The bladder is retroperitoneal and located behind the pubic symphysis in the pelvic floor.
In males, the bladder is situated in front of the rectum, while in females, it is positioned anterior to the vagina and uterus. The bladder floor contains an inverted triangular area called the trigone, defined by the two ureteric...
3.5K
Disorders of the Urinary System01:20

Disorders of the Urinary System

1.2K
The urinary system is responsible for eliminating waste and excess fluids from the body. However, disorders of the urinary system can arise due to various reasons like infections, stress, age, congenital abnormalities, and lifestyle.
Urinary tract infections (UTIs) are one of the most common urinary system disorders. They are caused by bacteria that enter the urethra and can spread to the bladder resulting in cystitis. Pyelonephritis is the result of a UTI that has ascended to the level of the...
1.2K
The Extracellular Matrix01:42

The Extracellular Matrix

89.2K
Overview
89.2K
The Extracellular Matrix01:29

The Extracellular Matrix

12.3K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
12.3K
Urinary Tract Calculi I: Introduction01:28

Urinary Tract Calculi I: Introduction

545
Renal calculi, or kidney stones, are solid deposits of minerals and salts formed inside the kidneys. In medical terminology, "calculus" refers to the stone itself, while "lithiasis" describes the process of stone formation. Depending on their location within the urinary system, these stones may be classified as either urolithiasis, when situated within the urinary tract, or nephrolithiasis, when located within the kidneys. Each term signifies the specific impact of the stone.Predisposition...
545

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Benzyl alcohol biosynthesis and its subcellular compartmentalization enable scent formation and salicylic acid production.

Nature communications·2026
Same author

A long-distance signaling loop promotes soybean nodulation and productivity.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Proteomic identification and validation of novel neuronal EV-based markers for Alzheimer's disease biomarker discovery.

bioRxiv : the preprint server for biology·2026
Same author

Coregulated metabolite networks associated with global protein crotonylation are central pathophysiological processes in prediabetes and diabetes.

medRxiv : the preprint server for health sciences·2026
Same author

Comprehensive Profiling of Protein N-Terminal Acetylation Stoichiometry in Extracellular Vesicles.

Analytical chemistry·2026
Same author

Author Correction: Discovery of chirally dependent protein modifications by D- and L-2-hydroxyglutarates.

Nature chemistry·2026

Related Experiment Video

Updated: Feb 7, 2026

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.7K

Highly Efficient Phosphoproteome Capture and Analysis from Urinary Extracellular Vesicles.

Xiaofeng Wu, Li Li1, Anton Iliuk1

  • 1Tymora Analytical Operations , West Lafayette , Indiana 47906 , United States.

Journal of Proteome Research
|August 7, 2018
PubMed
Summary

A novel EVTRAP method significantly improves extracellular vesicle (EV) isolation from urine, achieving over 95% recovery. This method enables comprehensive phosphoproteome analysis for cancer diagnostics.

Keywords:
EVTRAPEVsexosomesextracellular vesiclesphosphoproteomicsurine

More Related Videos

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
09:39

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

24.0K
Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles
09:30

Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles

Published on: March 31, 2020

8.2K

Related Experiment Videos

Last Updated: Feb 7, 2026

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.7K
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
09:39

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

24.0K
Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles
09:30

Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles

Published on: March 31, 2020

8.2K

Area of Science:

  • Biochemistry
  • Oncology
  • Biotechnology

Background:

  • Protein phosphorylation analysis in extracellular vesicles (EVs) is crucial for cancer signaling and early diagnosis.
  • Current EV isolation methods from biofluids suffer from low yield and impurity, hindering phosphoproteome analysis.

Purpose of the Study:

  • To assess various EV isolation methods, including a novel rapid method (EVTRAP).
  • To evaluate the efficiency and effectiveness of EVTRAP for isolating EVs from human urine for phosphoproteome analysis.

Main Methods:

  • Extensive assessment of multiple EV isolation techniques.
  • Application of the novel EVTRAP method for high-efficiency EV capture from human urine.
  • Proteomic and phosphoproteomic analysis of isolated EVs using mass spectrometry.

Main Results:

  • EVTRAP consistently achieved over 95% EV recovery yield, a significant improvement over standard methods.
  • EVTRAP identified over 2000 unique EV proteins and close to 2000 unique phosphopeptides from urine samples.
  • EVTRAP demonstrated superior recovery of known EV markers compared to ultracentrifugation.

Conclusions:

  • EVTRAP is a highly efficient and rapid method for isolating extracellular vesicles from human urine.
  • The method facilitates comprehensive EV phosphoproteome analysis, offering potential for clinical applications in early cancer diagnosis.