Related Experiment Video
Updated: Feb 7, 2026
10:13
Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
Published on: November 6, 2017
20.6K
Complexin in ivermectin resistance in body lice
Nadia Amanzougaghene1, Florence Fenollar2, Claude Nappez2
1Aix Marseille Univ, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Marseille, France.
Plos Genetics
|August 7, 2018
Summary
Ivermectin resistance in body lice is not due to target-site mutations. Instead, reduced expression of the neuronal protein complexin, caused by a gene mutation, significantly contributes to ivermectin resistance.
Area of Science:
- Entomology
- Molecular Biology
- Parasitology
Background:
- Ivermectin is a crucial pediculicide, but resistance is emerging.
- Field-evolved ivermectin resistance in body lice necessitates understanding resistance mechanisms.
Purpose of the Study:
- Investigate mechanisms of ivermectin resistance in body lice.
- Identify genetic and proteomic factors contributing to ivermectin resistance.
Main Methods:
- Analyzed mutations in the ivermectin target site (GluCl).
- Performed proteomic analysis on susceptible and resistant body lice.
- Utilized qRT-PCR to correlate mRNA and protein levels.
- Confirmed complexin's role using RNA interference.
Main Results:
- No non-silent mutations were found in the GluCl target site.
- Proteomic analysis revealed 22 differentially expressed proteins, with complexin significantly downregulated.
- A nonsense mutation in complexin was identified, potentially causing its reduced expression.
- Knocking down complexin expression induced ivermectin resistance in susceptible lice.
Conclusions:
- Complexin plays a significant role in ivermectin resistance in body lice.
- This study provides the first evidence linking complexin to insecticide resistance.
- Mechanisms of ivermectin resistance involve complexin downregulation, not target-site mutations.
Related Concept Videos
01:22Resistivity
4.6K
When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.6K
01:19Resistance
6.0K
When a current moves through any conductor, the conductor causes some level of difficulty for the current to flow. The measure of that difficulty is known as the resistance of the material and is represented by R. Every material has its own resistance. In the case of conductors, heat is emitted whenever a current passes through them. Resistance depends on the resistivity of the material. Resistivity is a characteristic of the material used to fabricate electrical components, whereas the...
6.0K
01:16Equivalent Resistance
987
In circuit analysis, situations often arise where resistors are neither in series nor parallel configurations. To tackle such scenarios, three-terminal equivalent networks like the wye (Y) (Figure 1 (a)) or tee (T) and delta (Δ) (Figure 1 (b)) or pi (π) networks come into play. These networks offer versatile solutions and are frequently encountered in various applications, including three-phase electrical systems, electrical filters, and matching networks.
987
01:25Resistance and Conductance
518
A conductor's DC resistance at a given temperature is influenced by its resistivity, length, and cross-sectional area. Resistivity is an inherent property of the conductor material, with annealed copper serving as the international standard for measurement. For instance, the resistivity of hard-drawn aluminum at 20 degrees Celsius is 61% of the standard conductivity of annealed copper.
Various factors impact the resistance of a conductor. Spiraling in stranded conductors increases their...
Various factors impact the resistance of a conductor. Spiraling in stranded conductors increases their...
518
01:21Rolling Resistance
669
When a solid cylinder rolls steadily on a rigid surface, the normal force applied by the surface on the cylinder is perpendicular to the tangent at the contact point. However, since no materials are entirely rigid, the surface's reaction to the cylinder involves a range of normal pressures.
For instance, imagine a hard cylinder rolling on a comparatively soft surface. The cylinder's weight compresses the surface beneath it. As the cylinder moves, the material in front of it slows down due to...
For instance, imagine a hard cylinder rolling on a comparatively soft surface. The cylinder's weight compresses the surface beneath it. As the cylinder moves, the material in front of it slows down due to...
669
01:20Vascular Resistance
11.1K
Vascular resistance is a critical concept in understanding blood flow dynamics in the circulatory system. It refers to the resistance that blood encounters as it flows through the blood vessels. This resistance is a key factor in determining blood pressure and cardiac workload.
The primary determinants of vascular resistance are vessel diameter, blood viscosity, and vessel length. Among these, vessel diameter plays the most significant role due to the fourth power relationship described by...
The primary determinants of vascular resistance are vessel diameter, blood viscosity, and vessel length. Among these, vessel diameter plays the most significant role due to the fourth power relationship described by...
11.1K