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Chitosan-alginate immobilized lipase based catalytic constructs: Development, characterization and potential
Robina Rashid1, Zahid Anwar1, Muddassar Zafar1
1Department of Biochemistry and Biotechnology, University of Gujrat, Pakistan.
International Journal of Biological Macromolecules
|August 7, 2018
Summary
This study optimized and purified lipase (LIP) from Aspergillus crevinus, immobilizing it onto chitosan-alginate beads. The immobilized lipase (CTS-ALG-LIPs) demonstrated enhanced stability and activity, proving effective for dehairing sheepskin in the leather industry.
Area of Science:
- Biochemistry
- Enzyme Technology
- Biomaterials
Background:
- Lipase (LIP) is a crucial enzyme with diverse industrial applications.
- Optimization and immobilization enhance enzyme stability and functionality.
- Developing efficient biocatalysts is key for sustainable industrial processes.
Purpose of the Study:
- To isolate, optimize, and purify lipase from Aspergillus crevinus.
- To immobilize the purified lipase onto chitosan-alginate (CTS-ALG) beads.
- To evaluate the properties and industrial applicability of the immobilized lipase.
Main Methods:
- Lipase isolation and purification using ammonium sulfate fractionation and Sephadex G-100 chromatography.
- Immobilization of lipase onto CTS-ALG beads using glutaraldehyde crosslinking.
- Characterization of free and immobilized lipase activity, stability (pH, temperature), kinetics, and performance in dehairing applications.
Main Results:
- Purified LIP showed a specific activity of 223.60 U/mg and a molecular mass of 60 kDa.
- Immobilized lipase (CTS-ALG-LIPs) exhibited broader optimal pH (5-11) and temperature (75°C) stability compared to free LIP.
- CTS-ALG-LIPs retained over 80% activity in the presence of inhibitors and achieved 100% dehairing of sheepskin within 30 minutes.
Conclusions:
- Immobilization onto CTS-ALG beads significantly enhanced lipase stability and activity.
- The developed immobilized lipase is a promising biocatalyst for the leather and tannery industry, particularly for dehairing applications.
- The retained conformational flexibility and stability of CTS-ALG-LIPs suggest their potential for various industrial uses.
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