Use of metal chlorides during waste wheat straw autohydrolysis to overcome the self-buffering effect

High ash content of waste wheat straw (WWS) is resistant to biorefinery autohydrolysis pretreatment due to its self-buffering effects. In this work, minor addition FeCl₃ and AlCl₃ were applied to overcome the self-buffering effects of WWS by cationic occupation of the negatively charged sites present on particulate ash's surface. The results showed that with the increasing concentrations (0-20 mM) of AlCl₃ and FeCl₃, the enzymatic efficiencies of autohydrolyzed WWS were enhanced from 49.7% to 62.1% and 66.6%, respectively. Acid buffer and cation exchange capacity of pretreated WWS were decreased by adding metal chlorides and the reducing results were mainly attributed to cation exchange. Meanwhile, a maximum monosaccharide production (185.3 mg/g-WWS) was achieved with 62.0 mg/g-WWS xylooligosaccharide by using 20 mM FeCl₃ during WWS autohydrolysis. The results demonstrated that the implications of FeCl₃ and AlCl₃ in WWS autohydrolysis were an effective strategy to enhance autohydrolysis efficiency by overcoming self-buffering effects.