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Updated: Feb 6, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
[Polyclonal antibody and immunomagnetic beads preparation technology for type A trichothecenes]
Gangtao Wu1, Yaling Wang1, Kexin Zhang1
1College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, China.
Objective:
Preparing immunomagnetic beads for enrichment and purification of mask type A trichothecenes.
Methods:
Through immunizing New Zealand white rabbits with family A toxin artificial antigen 3-Ac-NEOS-HS-BSA, the polyclonal antibodies of type A trichothecenes( type A family) were isolated, as well as its antibody titre were determinated and purified. The coupling condition of high titer antibody and immunomagnetic beads were optimized and applied.
Results:
After 5 times of immunization with type A trichothecene family toxin, the rabbit serum inhibited the highest antibody titer( 1 : 64 000). The optimal coupling condition of antibody and immunomagnetic beads were: temperature = 24 ℃, rotation capture in pH 7. 4, 0. 02 mol/L PBS buffer solution with 10% methanol in 24 h.
Conclusion:
The establishment of preparation method of type A single family toxin polyclonal antibody and immunomagnetic beads can make a standard for enrichment and purification of type A matrix complex single family toxins.
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