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Generation of Genetically Modified Mice through the Microinjection of Oocytes
Published on: June 15, 2017
[Three-generation study on neuroethology and cognitive ability of genetically modified maize with Cry1Ab and epsps
Mengfan Guo1, Chao Han1, Yan Li1
1Key Laboratory of Trace Element Nutrition, National Health and Family Planning Commission Institute for Nutrition and Health, China CDC, Beijing 100050, China.
Objective:
To evaluate the neurotoxicity effects of the genetically modified maize with insect-resistant Cry1 Ab and herbicide-resistant epsps genes on the SD rats through three generation exposure.
Methods:
180 weaning SD rats for F0 generation were divided into three groups, which were treated with parental maize diet( YC group), genetically modified maize diet( T group) and AIN-93 G control diet( C group). After three generations of breeding, 20 litters of rats those from pre-weaning F3 generation were selected randomly to evaluate early postnatal developmental neurotoxicity. After weaning, 10 pups were randomly selected from each litter( 1 male or female per litter) for future experiments of motor activity, cognitive function and neuropathology assessment as adult.
Results:
There were no statistically significant differences on early neurological development, neuroethology and cognitive ability. Only in the spatial probe test, the distance and time spent as well as their corresponding percentage in the target quadrant of YC group female rats were less compared with T group and C group( P < 0. 05).
Conclusion:
Under current experimental conditions, no adverse effects were found in neuroethology and cognitive ability on SD rats though the three generation study of genetically modified maize with Cry1Ab and epsps genes.
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