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Viral Nanoparticles for In vivo Tumor Imaging
Published on: November 16, 2012
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Lifetime-engineered NIR-II nanoparticles unlock multiplexed in vivo imaging
Yong Fan1, Peiyuan Wang1, Yiqing Lu2
1Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials and iChem, Fudan University, Shanghai, China.
Nature Nanotechnology
|August 8, 2018
Summary
Lanthanide-doped nanoparticles enable deep tissue imaging using engineered luminescence lifetimes for multiplexed diagnosis. This time-domain multiplexing approach overcomes signal attenuation for enhanced in vivo disease identification.
Area of Science:
- Biomedical Optics
- Nanotechnology
- Medical Imaging
Background:
- Deep tissue imaging in the second near-infrared (NIR-II) window offers significant potential for physiological studies and biomedical applications.
- Inhomogeneous signal attenuation in biological tissues limits the use of multiple-wavelength probes for multiplexed imaging.
- Current methods struggle with signal penetration and specificity in complex biological environments.
Purpose of the Study:
- To develop lanthanide-doped NIR-II nanoparticles with engineered luminescence lifetimes for in vivo quantitative imaging.
- To enable time-domain multiplexing for overcoming signal attenuation challenges in deep tissue imaging.
- To demonstrate the application of this technology for identifying tumour subtypes in living subjects.
Main Methods:
- Engineered lanthanide-doped NIR-II nanoparticles with tunable luminescence lifetimes using a controlled energy relay system.
- Developed a single emission band with a tunable lifetime range spanning three orders of magnitude.
- Utilized time-domain multiplexing for quantitative in vivo imaging and signal resolution at depths up to 8 mm.
Main Results:
- Successfully resolved selected nanoparticle probe lifetimes at depths of up to 8 mm in biological tissues, even where signal-to-noise ratios for intensity measurements were below 1.5.
- Demonstrated that lifetime coding is robust and independent of tissue penetration depth.
- Achieved in vivo multiplexing to accurately identify tumour subtypes in living mice, with results correlating well to ex vivo immunohistochemistry.
Conclusions:
- Lanthanide-doped NIR-II nanoparticles with engineered luminescence lifetimes provide a powerful tool for deep tissue quantitative imaging.
- Time-domain multiplexing using luminescence lifetime offers a robust and minimally invasive approach for in vivo disease diagnosis, such as tumour subtyping.
- This technology has the potential to significantly advance diagnostic capabilities in biomedical applications.
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