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Analysis of Demineralized Chemical Substances for Disinfecting Gutta-percha Cones
George Táccio de Miranda Candeiro1,2, Eduardo Akisue3, Fabrícia Campelo Correia4
1Department of Restorative Dentistry, Faculty of Dentistry, University of São Paulo, São Paulo, Brazil.
Introduction:
The aim of the present research was to evaluate the effectiveness of 5% malic acid, 17% EDTA and 10% citric acid solutions used to disinfect gutta-percha cones contaminated by Enterococcusfaecalis (ATCC 29212).
Methods And Materials:
Two hundred and ten previously sterilized gutta-percha cones were contaminated with E. faecalis at concentration of 1.5×108 CFU/mL. The cones were immersed in 5% malic acid, 17% EDTA, 10% citric acid, 1% NaOCl and 2.5% NaOCl for 1, 5 and 10 min. Then each cone was kept in Eppendorf tubes containing BHI sterile solution at 37°C for 48 h. The presence of turbidity in BHI solution was analyzed. The results were statistically analyzed by Kruskal-Wallis test and 5% Dunn comparisons. P-value was considered statistically significant when P<0.05.
Results:
Regardless of exposure time, 1% NaOCl and 2.5% NaOCl were the most effective agents for rapid disinfection of gutta-percha cones (P<0.001). All specimens immersed in experimental demineralized solutions presented bacterial growth (P>0.05).
Conclusion:
Demineralized solutions tested were not effective for elimination of Enterococcus faecalis on the surface of gutta-percha cones.
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