A Dual-Target Fluorescent Probe with Response-Time Dependent Selectivity for Cd2+ and Cu2
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Updated: Feb 6, 2026

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
Published on: June 26, 2010
Zhengyu Zhang1, Shizhuang Yuan1, Enju Wang2
1Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry & Chemical Engineering, Hainan Normal University, Haikou, 571158, China.
Abstract:
A novel fluorescent probe (NT) was developed by merging 2-hydrazinylbenzothiazole with 2-hydroxy-1-naphthaldehyde for the detection of Cd2+ and Cu2+. The probe alone is almost nonfluorescent due to the isomerization of C=N in the excited state. The addition of Cd2+ can cause an immediate strong green fluorescence owing to the suppression of C=N isomerization by Cd2+-coordination. Furthermore, NT gives a delayed turn-on fluorescence response to Cu2+ although it is a vigorous fluorescence quencher, which was thanks to the inhibition of the electron transfer between excited fluorophore and paramagnetic Cu2+ by sulfur donor. Based on fluorescence spectra and ESI-MS analysis, the binding modes between NT and Cd2+/Cu2+ were proposed.
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