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Extraction and Detection of Geosmin and 2-Methylisoborneol in Water and Fish using High-Capacity Sorptive Extraction Probes and GC-MS
Published on: July 3, 2025
Capacity Building through Water Quality and Safety Analyses in Herat, Afghanistan
Paul D Ebner1, Amanda Deering2, Mosa Mojadady3
11 Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
Abstract:
Contaminated water is a leading cause of approximately 600 million annual cases of foodborne disease globally. Twenty percent of all child mortality in Afghanistan is attributed to diarrheal diseases. There are limited data on water quality and safety in Afghanistan as well as limited laboratory capacity for food and water analyses. The purpose of this study was to conduct a regional water assessment study and, in doing so, train the first class of food technology undergraduate students at Herat University (Herat City, Afghanistan) in basic water quality and safety laboratory techniques. In total, 235 water samples from private wells ( n = 128) and municipal water system taps ( n = 107) were collected from Herat Province, Afghanistan. Samples were aseptically collected, transferred, and analyzed at the Herat University Food Technology Laboratory for nitrate, nitrite, lead, phosphate, and arsenic concentrations; hardness; total coliforms; and Escherichia coli. We did not detect arsenic in any sample tested ( n = 234), and lead levels in samples tested ( n = 28) were below the U.S. Environmental Protection Agency maximum contaminant level (15 μg/L). In contrast, 38 of 232 samples had nitrate (NO3) levels greater than the maximum contaminant level (10 mg/L) and 15 of 232 samples had nitrite (NO2) levels > 0.3 mg/L. On average, well water was harder than municipal water; there were no differences in phosphate (PO4) levels. Furthermore, 93 (43.9%) of 212 samples had detectable coliforms (average CFU/100 mL) and 52 (24.3%) of 214 samples had detectable E. coli (average 28.6 CFU/100 mL). E. coli was detected in 21.4 and 26.7% of municipal and well water samples, respectively. This study indicates a clear need for systematic analyses of Herat City water to develop plans for water quality and safety improvement and management. The students engaged in the research project now have the basic research and analytical skills needed to address water and foodborne disease issues endemic in the area.
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One key metric is the Inspiratory Capacity (IC), which represents the maximum amount of air that can be inhaled with full effort. IC is calculated by summing the tidal volume and inspiratory reserve volume, typically ranging from 2.4 to 3.6 liters.
The Functional Residual Capacity (FRC) represents the air in the...

