Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Hormone Secretion01:19

Regulation of Hormone Secretion

6.9K
Regulation of hormone secretion is a finely tuned orchestration driven by various types of stimuli, encompassing neural, humoral, and hormonal signals. Environmental cues instigate neural stimuli, where action potentials traverse nerve fibers to reach their designated targets. An illustrative scenario is the body's response to stress, wherein the sympathetic nervous system releases epinephrine from the adrenal glands, inducing the well-known 'fight or flight' reaction.
Humoral...
6.9K
Hormones Secreted by the Stomach01:25

Hormones Secreted by the Stomach

2.7K
Enteroendocrine cells, accounting for only 1% of stomach epithelial cells, play a significant role in digestion and are classified by their digestive hormone secretions.
Each of these hormones secreted by different enteroendocrine cells plays a unique role in digestion. Here are a few examples:
2.7K
Pancreatic Juice and Secretion01:26

Pancreatic Juice and Secretion

3.1K
Pancreatic juice is a clear fluid produced by the pancreas, containing water, salts, sodium bicarbonate, and enzymes vital for digestion in the small intestine. It helps break down large molecules, facilitating nutrient absorption.
When acidic chyme from the stomach enters the duodenum, it triggers the release of secretin, a hormone that prompts pancreatic juice secretion. After a fatty meal, cholecystokinin, another hormone, stimulates gallbladder contraction and enhances enzyme-rich...
3.1K
Tubular Reabsorption and Secretion01:28

Tubular Reabsorption and Secretion

6.8K
Tubular secretion and reabsorption are two critical processes in the nephron tubule of the kidneys. When the fluid filtered from the glomerulus enters the proximal convoluted tubule, it is referred to as filtrate, and its composition changes due to tubular reabsorption and secretion.
Tubular reabsorption is a selective process that starts when the filtrate enters the proximal tubules. It involves substances traveling through the transcellular route (through the tubule cell and peritubular...
6.8K
Reabsorption and Secretion in the PCT01:28

Reabsorption and Secretion in the PCT

3.6K
The Proximal Convoluted Tubule, or PCT, plays a pivotal role in the body's filtration system. They are primarily responsible for reabsorbing solutes and water from the filtered fluid produced by the glomeruli. Most of the filtered water, ions, and organic solutes such as glucose and amino acids are reabsorbed by the PCT.
Transport mechanisms involving sodium ions (Na+) contribute significantly to solute reabsorption. These mechanisms include symport and antiport processes.
A key example is the...
3.6K
Cells and Secretions of the Pancreas01:16

Cells and Secretions of the Pancreas

5.3K
The pancreas, a vital organ within the abdominal cavity, plays dual roles in the digestive and endocrine systems, collaborating with exocrine and endocrine cells to maintain optimal digestion and blood sugar levels.
Exocrine function is carried out by acinar cells, organized into clusters known as acini. These cells contribute to digestion by releasing substantial quantities of enzyme-rich, alkaline digestive juices.
Concurrently, the dispersed clusters of endocrine cells throughout the...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Soluble multi-epitope protein vaccination leverages antigen availability to drive CD8<sup>+</sup> T cell immunity and tumor control.

Frontiers in immunology·2026
Same author

Tracking spatio-temporal dynamics of early immune responses to an intranasal OMV-based pneumococcal vaccine candidate in mice.

NPJ vaccines·2026
Same author

Verapamil Suppresses the Development of Resistance Against Anti-Tuberculosis Drugs in Mycobacteria.

International journal of molecular sciences·2025
Same author

Overproduction of β-barrel outer membrane proteins in <i>Escherichia coli</i> BL21(DE3) induces hypervesiculation.

Extracellular vesicles and circulating nucleic acids·2025
Same author

Impact of expression systems on the transcriptome of <i>Bacillus subtilis</i>: insights for enhanced production of glutaminase PrgA.

Applied and environmental microbiology·2025
Same author

An Rcs Stress-Based High-Throughput Screen Reveals Novel Gyrase Inhibitors as Indirect Inducers of Cell Envelope Stress in Gram-Negative Bacteria.

ACS infectious diseases·2025

Related Experiment Video

Updated: Feb 6, 2026

Author Spotlight: Advancing Bioimaging and Therapy with Functional Nanomaterials
07:12

Author Spotlight: Advancing Bioimaging and Therapy with Functional Nanomaterials

Published on: September 13, 2024

3.0K

On display: autotransporter secretion and application.

Peter van Ulsen1, Katinka M Zinner1, Wouter S P Jong2

  • 1Section Molecular Microbiology, Department of Molecular Cell Biology, Amsterdam Institute for Molecules, Medicines and Systems (AIMMS), Vrije Universiteit, 1081 HV Amsterdam, The Netherlands.

FEMS Microbiology Letters
|August 8, 2018
PubMed
Summary

Gram-negative bacteria use autotransporters to export proteins. This review details autotransporter secretion mechanisms and their use in displaying proteins on cell surfaces for vaccines and biotechnology.

More Related Videos

Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate
05:58

Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate

Published on: June 25, 2019

8.0K
Recording Ultra-Realistic Full-Color Analog Holograms for Use in a Moving Hologram Display
09:04

Recording Ultra-Realistic Full-Color Analog Holograms for Use in a Moving Hologram Display

Published on: January 14, 2020

10.3K

Related Experiment Videos

Last Updated: Feb 6, 2026

Author Spotlight: Advancing Bioimaging and Therapy with Functional Nanomaterials
07:12

Author Spotlight: Advancing Bioimaging and Therapy with Functional Nanomaterials

Published on: September 13, 2024

3.0K
Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate
05:58

Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate

Published on: June 25, 2019

8.0K
Recording Ultra-Realistic Full-Color Analog Holograms for Use in a Moving Hologram Display
09:04

Recording Ultra-Realistic Full-Color Analog Holograms for Use in a Moving Hologram Display

Published on: January 14, 2020

10.3K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Monomeric autotransporters are essential for Gram-negative bacteria to export proteins involved in virulence and colonization.
  • These proteins traverse the inner and outer membranes via the Sec translocon and Bam complex, respectively.

Purpose of the Study:

  • To review the mechanisms of autotransporter translocation across the outer membrane.
  • To discuss the application of autotransporters for heterologous protein display, particularly in vaccine development.
  • To introduce novel strategies for overcoming secretion limitations.

Main Methods:

  • Analysis of autotransporter secretion pathways.
  • Highlighting interactions with the Bam complex.
  • Reviewing structural data of the Bam complex to propose a mechanistic model.
  • Summarizing recent advancements in autotransporter applications.
  • Presenting new ligation technologies for protein display.

Main Results:

  • Autotransporter secretion involves sequential passage through the inner and outer membranes.
  • The structure of the Bam complex provides insights into a mechanistic model for autotransporter secretion.
  • Autotransporter systems are effective for surface display of proteins, including in outer membrane vesicle vaccines.
  • Limitations exist for secreting complex heterologous proteins.
  • A new technology allows ligation of proteins to cell-surface displayed autotransporters.

Conclusions:

  • Understanding autotransporter secretion mechanisms, particularly interactions with the Bam complex, is crucial.
  • Autotransporter systems offer significant potential for biotechnological and biomedical applications like vaccine development.
  • New ligation technologies can overcome existing limitations in secreting complex proteins using autotransporter platforms.