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DNA methylation patterns of the S100A14, POU2F3 and SFN genes in equine sarcoid tissues

E Semik-Gurgul1, T Ząbek1, A Fornal1

  • 1National Research Institute of Animal Production, Department of Animal Genomics and Molecular Biology, Krakowska 1, 32-083 Balice, Poland.

Research in Veterinary Science
|August 8, 2018
PubMed

Insights

Equine sarcoid tumors show increased DNA methylation in the S100A14A gene promoter region. Further research is needed to understand the role of aberrant methylation in equine sarcoid progression.

Area of Science:

  • Veterinary Oncology
  • Epigenetics
  • Equine Dermatology

Background:

  • Equine sarcoids are locally invasive skin tumors in horses.
  • Genetic and epigenetic alterations, particularly in tumor suppressor genes, are implicated in cancer development.
  • The role of DNA methylation in equine sarcoid progression is not well understood.

Purpose of the Study:

  • To investigate DNA methylation levels in the promoter regions of SFN, S100A14, and POU2F3 genes in equine sarcoid samples.
  • To determine if DNA methylation correlates with altered gene expression during tumor progression.

Main Methods:

  • Bisulfite sequencing and clone sequencing were employed.
  • Methylation levels in promoter regions of SFN, S100A14, and POU2F3 were analyzed.
  • Lesional sarcoid samples were compared with corresponding normal skin tissue.

Main Results:

  • Significantly higher DNA methylation rates were observed in the S100A14A promoter region of lesional sarcoid samples compared to normal skin.
  • Frequent methylation of SFN and POU2F3 promoter sequences was detected in both tumor and control tissues.
  • Aberrant methylation patterns may contribute to altered gene expression in equine sarcoids.

Conclusions:

  • DNA methylation in the S100A14A promoter is associated with equine sarcoid development.
  • Further investigation is required to elucidate the precise role of aberrant methylation in sarcoid progression.
  • Understanding these epigenetic mechanisms could lead to novel therapeutic strategies for equine sarcoids.
Keywords:
DNA methylationEquine sarcoidsPOU2F3S100A14SFNTumorigenesis

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